As synthetic biology permeates society, the signal processing circuits in engineered living
systems must be customized to meet practical demands. Towards this mission, novel …

Predictive biology is the next great chapter in synthetic and systems biology, particularly for
microorganisms. Tasks that once seemed infeasible are increasingly being realized such as …

In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

Transcription induces a wave of DNA supercoiling, altering the binding affinity of RNA
polymerases and resha** the biochemical landscape of gene regulation. As supercoiling …

Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …

Gene expression control based on CRISPRi (clustered regularly interspaced short
palindromic repeats interference) has emerged as a powerful tool for creating synthetic gene …

A longstanding goal of synthetic biology has been the programmable control of cellular
functions. Central to this is the creation of versatile regulatory toolsets that allow for …

Cells provide dynamic platforms for executing exogenous genetic programs in synthetic
biology, resulting in highly context-dependent circuit performance. Recent years have seen …

Synthetic biology has the potential to bring forth advanced genetic devices for applications
in healthcare and biotechnology. However, accurately predicting the behavior of engineered …

The l-arabinose-responsive AraC and its cognate PBAD promoter underlie one of the most
often used chemically inducible prokaryotic gene expression systems in microbiology and …