Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques

R Amann, BM Fuchs - Nature Reviews Microbiology, 2008 - nature.com
The ribosomal-RNA (rRNA) approach to microbial evolution and ecology has become an
integral part of environmental microbiology. Based on the patchy conservation of rRNA …

Metagenomics: DNA sequencing of environmental samples

SG Tringe, EM Rubin - Nature reviews genetics, 2005 - nature.com
Although genomics has classically focused on pure, easy-to-obtain samples, such as
microbes that grow readily in culture or large animals and plants, these organisms represent …

Archaeal nitrification in the ocean

C Wuchter, B Abbas, MJL Coolen… - Proceedings of the …, 2006 - National Acad Sciences
Marine Crenarchaeota are the most abundant single group of prokaryotes in the ocean, but
their physiology and role in marine biogeochemical cycles are unknown. Recently, a …

phyloFlash: rapid small-subunit rRNA profiling and targeted assembly from metagenomes

HR Gruber-Vodicka, BKB Seah, E Pruesse - Msystems, 2020 - Am Soc Microbiol
The small-subunit rRNA (SSU rRNA) gene is the key marker in molecular ecology for all
domains of life, but it is largely absent from metagenome-assembled genomes that often are …

[BOOK][B] Microwave-assisted extraction for bioactive compounds: theory and practice

F Chemat, G Cravotto - 2012 - books.google.com
With increasing energy prices and the drive to reduce CO2 emissions, food industries are
challenged to find new technologies in order to reduce energy consumption, to meet legal …

The future is now: single-cell genomics of bacteria and archaea

PC Blainey - FEMS microbiology reviews, 2013 - academic.oup.com
Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of
heterogeneity among seemingly similar cells, and technological advances in whole-genome …

A bioinformatician's guide to metagenomics

V Kunin, A Copeland, A Lapidus… - Microbiology and …, 2008 - Am Soc Microbiol
As random shotgun metagenomic projects proliferate and become the dominant source of
publicly available sequence data, procedures for the best practices in their execution and …

Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities

S Müller, G Nebe-von-Caron - FEMS Microbiology reviews, 2010 - academic.oup.com
The still poorly explored world of microbial functioning is about to be uncovered by a
combined application of old and new technologies. Bacteria, especially, are still in the dark …

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

G Batani, K Bayer, J Böge, U Hentschel, T Thomas - Scientific reports, 2019 - nature.com
Despite the development of several cultivation methods, the rate of discovery of
microorganisms that are yet-to-be cultivated outpaces the rate of isolating and cultivating …

Metagenome-assembled genomes uncover a global brackish microbiome

LW Hugerth, J Larsson, J Alneberg, MV Lindh… - Genome biology, 2015 - Springer
Background Microbes are main drivers of biogeochemical cycles in oceans and lakes.
Although the genome is a foundation for understanding the metabolism, ecology and …