The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …

▪ Abstract Multistep chemical reactions are increasingly seen as important in a growing
number of complex biotransformations. Covalently attached prosthetic groups or swinging …

Ideally, therapy for autoimmune diseases should eliminate pathogenic autoimmune cells
while sparing protective immunity, but feasible strategies for such an approach have been …

Proteomic approaches require simple and efficient protein purification methodologies that
are amenable to high throughput. Biotinylation is an attractive approach for protein complex …

Protein production for structural and biophysical studies, functional assays, biomarkers,
mechanistic studies in vitro and in vivo, but also for therapeutic applications in pharma …

Protein phosphorylation is a prevalent and ubiquitous mechanism of regulation. Kinases are
popular drug targets, but identifying selective phosphatase inhibitors has been challenging …

Biotin is a coenzyme essential to all life forms. The vitamin has biological activity only when
covalently attached to certain key metabolic enzymes. Most organisms have only one …

Publisher Summary This chapter discusses the biotinylation of proteins in vivo and in vitro
using small peptide tags. Proteins containing the AviTag can be efficiently biotinylated in …

The detection and characterization of fragment binding requires the use of technologies with
extreme sensitivity to observe the binding interactions of low-affinity and low-molecular …

Bacteriophages are viruses whose ubiquity in nature and remarkable specificity to their host
bacteria enable an impressive and growing field of tunable biotechnologies in agriculture …