Phosphoinositides are signalling lipids derived from phosphatidylinositol, a ubiquitous
phospholipid in the cytoplasmic leaflet of eukaryotic membranes. Initially discovered for their …

Lysosomes are degradation centers and signaling hubs in cells and play important roles in
cellular homeostasis, development, and aging. Changes in lysosome function are essential …

Cellular membranes can form two principally different involutions, which either exclude or
contain cytosol. The 'classical'budding reactions, such as those occurring during …

Autophagosome biogenesis involves de novo formation of a membrane that elongates to
sequester cytoplasmic cargo and closes to form a double-membrane vesicle (an …

Multivesicular bodies are key endosomal compartments implicated in cellular quality control
through their degradation of membrane-bound cargo proteins,–. The ATP-consuming …

We examined the luminal pH of individual lysosomes using quantitative ratiometric
fluorescence microscopy and report an unappreciated heterogeneity: peripheral lysosomes …

Cells operate through protein interaction networks organized in space and time. Here, we
describe an approach to resolve both dimensions simultaneously by using proximity …

Autophagy is the primary cellular catabolic program activated in response to nutrient
starvation. Initiation of autophagy, particularly by amino-acid withdrawal, requires the ULK …

Cells rely on autophagy to clear misfolded proteins and damaged organelles to maintain
cellular homeostasis. In this study we use the new autophagy inhibitor PIK-III to screen for …

Of the approximately 70 human Rab GTPases, nearly three-quarters are involved in
endocytic trafficking. Significant plasticity in endosomal membrane transport pathways is …