Small-molecule fluorescent probes for live-cell super-resolution microscopy
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …
cellular structures at the nanometer scale. Employing these techniques in living cells has …
Super-resolving microscopy in neuroscience
Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …
system. The emergence of various super-resolution microscopy methods and specialized …
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
Dense 4D nanoscale reconstruction of living brain tissue
Abstract Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function …
synapse level would create opportunities for decoding the dynamics and structure–function …
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
WWY Yim, H Yamamoto, N Mizushima - Elife, 2022 - elifesciences.org
Monitoring autophagic flux is necessary for most autophagy studies. The autophagic flux
assays currently available for mammalian cells are generally complicated and do not yield …
assays currently available for mammalian cells are generally complicated and do not yield …
Imaging brain tissue architecture across millimeter to nanometer scales
Map** the complex and dense arrangement of cells and their connectivity in brain tissue
demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels …
demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels …
MINFLUX fluorescence nanoscopy in biological tissue
T Moosmayer, KA Kiszka, V Westphal, JK Pape… - Proceedings of the …, 2024 - pnas.org
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly
sought-after goal, as it would provide visualization in physiologically relevant contexts …
sought-after goal, as it would provide visualization in physiologically relevant contexts …
Alternative neural systems: what is a neuron?(ctenophores, sponges and placozoans)
How to make a neuron, a synapse, and a neural circuit? Is there only one 'design'for a
neural architecture with a universally shared genomic blueprint across species? The brief …
neural architecture with a universally shared genomic blueprint across species? The brief …
Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging
F De Moliner, Z Konieczna… - Angewandte Chemie …, 2023 - Wiley Online Library
The multiple applications of super‐resolution microscopy have prompted the need for
minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many …
minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many …
[HTML][HTML] A brain atlas of synapse protein lifetime across the mouse lifespan
E Bulovaite, Z Qiu, M Kratschke, A Zgraj, DG Fricker… - Neuron, 2022 - cell.com
The lifetime of proteins in synapses is important for their signaling, maintenance, and
remodeling, and for memory duration. We quantified the lifetime of endogenous PSD95, an …
remodeling, and for memory duration. We quantified the lifetime of endogenous PSD95, an …