Small-molecule fluorescent probes for live-cell super-resolution microscopy

L Wang, MS Frei, A Salim… - Journal of the American …, 2018 - ACS Publications
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Super-resolving microscopy in neuroscience

C Werner, M Sauer, C Geis - Chemical Reviews, 2021 - ACS Publications
Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …

MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells

KC Gwosch, JK Pape, F Balzarotti, P Hoess… - Nature …, 2020 - nature.com
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …

Dense 4D nanoscale reconstruction of living brain tissue

P Velicky, E Miguel, JM Michalska, J Lyudchik, D Wei… - Nature …, 2023 - nature.com
Abstract Three-dimensional (3D) reconstruction of living brain tissue down to an individual
synapse level would create opportunities for decoding the dynamics and structure–function …

A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag

WWY Yim, H Yamamoto, N Mizushima - Elife, 2022 - elifesciences.org
Monitoring autophagic flux is necessary for most autophagy studies. The autophagic flux
assays currently available for mammalian cells are generally complicated and do not yield …

Imaging brain tissue architecture across millimeter to nanometer scales

JM Michalska, J Lyudchik, P Velicky… - Nature …, 2024 - nature.com
Map** the complex and dense arrangement of cells and their connectivity in brain tissue
demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels …

MINFLUX fluorescence nanoscopy in biological tissue

T Moosmayer, KA Kiszka, V Westphal, JK Pape… - Proceedings of the …, 2024 - pnas.org
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly
sought-after goal, as it would provide visualization in physiologically relevant contexts …

Alternative neural systems: what is a neuron?(ctenophores, sponges and placozoans)

LL Moroz, DY Romanova - Frontiers in Cell and Developmental …, 2022 - frontiersin.org
How to make a neuron, a synapse, and a neural circuit? Is there only one 'design'for a
neural architecture with a universally shared genomic blueprint across species? The brief …

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

F De Moliner, Z Konieczna… - Angewandte Chemie …, 2023 - Wiley Online Library
The multiple applications of super‐resolution microscopy have prompted the need for
minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many …

[HTML][HTML] A brain atlas of synapse protein lifetime across the mouse lifespan

E Bulovaite, Z Qiu, M Kratschke, A Zgraj, DG Fricker… - Neuron, 2022 - cell.com
The lifetime of proteins in synapses is important for their signaling, maintenance, and
remodeling, and for memory duration. We quantified the lifetime of endogenous PSD95, an …