Astrocytes are a type of glial cell that tile the CNS. They interact with multiple cell types,
including neurons, glial cells and blood vessels, and are involved or implicated in brain …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Adipocytes undergo intense energetic stress in obesity resulting in loss of mitochondrial
mass and function. We have found that adipocytes respond to mitochondrial stress by …

An essential step toward understanding brain function is to establish a structural framework
with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and …

We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …

Persistent neural activity maintains information that connects past and future events. Models
of persistent activity often invoke reverberations within local cortical circuits, but long-range …

Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

We describe the development and application of methods for high-throughput
neuroanatomy in Drosophila using light microscopy. These tools enable efficient multicolor …

Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

Visual projection neurons (VPNs) provide an anatomical connection between early visual
processing and higher brain regions. Here we characterize lobula columnar (LC) cells, a …