Precision genome editing in the CRISPR era

J Salsman, G Dellaire - Biochemistry and cell biology, 2017 - cdnsciencepub.com
With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …

Modeling Alzheimer's disease with human induced pluripotent stem (iPS) cells

AE Mungenast, S Siegert, LH Tsai - Molecular and Cellular Neuroscience, 2016 - Elsevier
In the last decade, induced pluripotent stem (iPS) cells have revolutionized the utility of
human in vitro models of neurological disease. The iPS-derived and differentiated cells …

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

D Paquet, D Kwart, A Chen, A Sproul, S Jacob, S Teo… - Nature, 2016 - nature.com
The bacterial CRISPR/Cas9 system allows sequence-specific gene editing in many
organisms and holds promise as a tool to generate models of human diseases, for example …

A prudent path forward for genomic engineering and germline gene modification

D Baltimore, P Berg, M Botchan, D Carroll, RA Charo… - Science, 2015 - science.org
Genome engineering technology offers unparalleled potential for modifying human and
nonhuman genomes. In humans, it holds the promise of curing genetic disease, while in …

RS-1 enhances CRISPR/Cas9-and TALEN-mediated knock-in efficiency

J Song, D Yang, J Xu, T Zhu, YE Chen… - Nature communications, 2016 - nature.com
Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered
regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are …

Defining the epoch we live in

WF Ruddiman, EC Ellis, JO Kaplan, DQ Fuller - Science, 2015 - science.org
Human alterations of Earth's environments are pervasive. Visible changes include the built
environment, conversion of forests and grasslands to agriculture, algal blooms, smog, and …

An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

J Cao, L Wu, SM Zhang, M Lu… - Nucleic acids …, 2016 - academic.oup.com
The CRISPR/Cas9 system is a powerful genome editing tool and has been widely used for
biomedical research. However, many challenges, such as off-target effects and lack of easy …

Developmental chromatin programs determine oncogenic competence in melanoma

A Baggiolini, SJ Callahan, E Montal, JM Weiss, T Trieu… - Science, 2021 - science.org
INTRODUCTION Tumor analyses have shown that DNA mutations can transform cells in
some situations but not in others. In melanoma, BRAFV600E [serine/threonine-protein …

TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells

N Verma, H Pan, LC Doré, A Shukla, QV Li… - Nature …, 2018 - nature.com
Abstract TET enzymes oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC),
which can lead to DNA demethylation. However, direct connections between TET-mediated …

Antisense lncRNA transcription mediates DNA demethylation to drive stochastic protocadherin α promoter choice

D Canzio, CL Nwakeze, A Horta, SM Rajkumar… - Cell, 2019 - cell.com
Stochastic activation of clustered Protocadherin (Pcdh) α, β, and γ genes generates a cell-
surface identity code in individual neurons that functions in neural circuit assembly. Here, we …