[HTML][HTML] Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology
A Rohman, BW Dijkstra - Biotechnology Advances, 2021 - Elsevier
Ketosteroid Δ 1-dehydrogenase catalyzes the 1 (2)-dehydrogenation of 3-ketosteroid
substrates using flavin adenine dinucleotide as a cofactor. The enzyme plays a crucial role …
substrates using flavin adenine dinucleotide as a cofactor. The enzyme plays a crucial role …
[HTML][HTML] The role and mechanism of microbial 3-ketosteroid Δ1-dehydrogenases in steroid breakdown
A Rohman, BW Dijkstra - The Journal of Steroid Biochemistry and …, 2019 - Elsevier
Ketosteroid Δ 1-dehydrogenases are FAD-dependent enzymes that catalyze the introduction
of a double bond between the C1 and C2 atoms of the A-ring of 3-ketosteroid substrates …
of a double bond between the C1 and C2 atoms of the A-ring of 3-ketosteroid substrates …
Highly Efficient Enzymatic Synthesis of C6-Substituted Δ1-3-Ketosteroids Enabled by Finely Tuning the Substrate Binding of 3-Ketosteroid-Δ1-Dehydrogenase
Y Wang, R Zhang, X Chen, Z Yang, J Feng… - ACS Sustainable …, 2024 - ACS Publications
Enzymatic Δ1-dehydrogenation mediated by 3-ketosteroid-Δ1-dehydrogenases (Δ1-KstDs,
EC 1.3. 99.4) offers an attractive method to access pharmaceutically important Δ1-3 …
EC 1.3. 99.4) offers an attractive method to access pharmaceutically important Δ1-3 …
iTRAQ-Based Quantitative Proteomic Analysis of Arthrobacter simplex in Response to Cortisone Acetate and Its Mutants with Improved Δ1-Dehydrogenation …
Y Wu, J Ma, J Shi, S Cao, J Luo, T Zheng… - Journal of Agricultural …, 2023 - ACS Publications
Arthrobacter simplex is extensively used for cortisone acetate (CA) biotransformation in
industry, but the Δ1-dehydrogenation molecular fundamental remains unclear. Herein, the …
industry, but the Δ1-dehydrogenation molecular fundamental remains unclear. Herein, the …
Crystal structure and site-directed mutagenesis of 3-ketosteroid Δ1-dehydrogenase from Rhodococcus erythropolis SQ1 explain its catalytic mechanism
A Rohman, N Van Oosterwijk… - Journal of Biological …, 2013 - ASBMB
3-Ketosteroid Δ 1-dehydrogenases are FAD-dependent enzymes that catalyze the 1, 2-
desaturation of 3-ketosteroid substrates to initiate degradation of the steroid nucleus. Here …
desaturation of 3-ketosteroid substrates to initiate degradation of the steroid nucleus. Here …
Enzymatic Δ1-Dehydrogenation of 3-Ketosteroids—Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism
M Glanowski, P Wójcik, M Procner, T Borowski… - ACS …, 2021 - ACS Publications
Δ1-Dehydrogenation of 3-ketosteroids catalyzed by flavin adenine dinucleotide (FAD)-
dependent 3-ketosteroid dehydrogenases (Δ1-KSTD) is a crucial step in steroid degradation …
dependent 3-ketosteroid dehydrogenases (Δ1-KSTD) is a crucial step in steroid degradation …
Identification, Biological Characteristics, and Active Site Residues of 3-Ketosteroid Δ1-Dehydrogenase Homologues from Arthrobacter simplex
JM Luo, HL Cui, HC Jia, F Li, HJ Cheng… - Journal of Agricultural …, 2020 - ACS Publications
3-Ketosteroid Δ1-dehydrogenase (KsdD) is the key enzyme responsible for Δ1-
dehydrogenation, which is one of the most valuable reactions for steroid catabolism …
dehydrogenation, which is one of the most valuable reactions for steroid catabolism …
Focused mutagenesis in non-catalytic cavity for improving catalytic efficiency of 3-ketosteroid-Δ1-dehydrogenase
Y Zhang, M Liu, H Wang, J Lin, F Chen - Molecular Catalysis, 2022 - Elsevier
Non-catalytic sites are commonly present in many enzymes, but how to classify and
modulate such sites for activity improvement is not well-explored. Herein a non-catalytic …
modulate such sites for activity improvement is not well-explored. Herein a non-catalytic …
Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
Background Biosynthesis of steroidal drugs is of great benefit in pharmaceutical
manufacturing as the process involves efficient enzymatic catalysis at ambient temperature …
manufacturing as the process involves efficient enzymatic catalysis at ambient temperature …
Universal capability of 3-ketosteroid Δ1-dehydrogenases to catalyze Δ1-dehydrogenation of C17-substituted steroids
P Wójcik, M Glanowski, AM Wojtkiewicz… - Microbial Cell …, 2021 - Springer
Abstract Background 3-Ketosteroid Δ1-dehydrogenases (KSTDs) are the enzymes involved
in microbial cholesterol degradation and modification of steroids. They catalyze …
in microbial cholesterol degradation and modification of steroids. They catalyze …