Spatial proteomics: a powerful discovery tool for cell biology

E Lundberg, GHH Borner - Nature Reviews Molecular Cell Biology, 2019 - nature.com
Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

Application of machine learning in spatial proteomics

M Mou, Z Pan, M Lu, H Sun, Y Wang… - Journal of Chemical …, 2022 - ACS Publications
Spatial proteomics is an interdisciplinary field that investigates the localization and dynamics
of proteins, and it has gained extensive attention in recent years, especially the subcellular …

Deep learning a boon for biophotonics?

P Pradhan, S Guo, O Ryabchykov, J Popp… - Journal of …, 2020 - Wiley Online Library
This review covers original articles using deep learning in the biophotonic field published in
the last years. In these years deep learning, which is a subset of machine learning mostly …

YeastSpotter: accurate and parameter-free web segmentation for microscopy images of yeast cells

AX Lu, T Zarin, IS Hsu, AM Moses - Bioinformatics, 2019 - academic.oup.com
We introduce YeastSpotter, a web application for the segmentation of yeast microscopy
images into single cells. YeastSpotter is user-friendly and generalizable, reducing the …

Integrative phenomics reveals insight into the structure of phenotypic diversity in budding yeast

DA Skelly, GE Merrihew, M Riffle, CF Connelly… - Genome …, 2013 - genome.cshlp.org
To better understand the quantitative characteristics and structure of phenotypic diversity, we
measured over 14,000 transcript, protein, metabolite, and morphological traits in 22 …

[HTML][HTML] Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast

X Cao, S Yang, C Cao, YJ Zhou - Synthetic and systems biotechnology, 2020 - Elsevier
Current yeast metabolic engineering in isoprenoids production mainly focuses on rewiring of
cytosolic metabolic pathway. However, the precursors, cofactors and the enzymes are …

CYCLoPs: a comprehensive database constructed from automated analysis of protein abundance and subcellular localization patterns in Saccharomyces cerevisiae

JLY Koh, YT Chong, H Friesen, A Moses… - G3: Genes …, 2015 - academic.oup.com
Abstract Changes in protein subcellular localization and abundance are central to biological
regulation in eukaryotic cells. Quantitative measures of protein dynamics in vivo are …

[HTML][HTML] Nuclear mRNA metabolism drives selective basket assembly on a subset of nuclear pore complexes in budding yeast

P Bensidoun, T Reiter, B Montpetit, D Zenklusen… - Molecular cell, 2022 - cell.com
To determine which transcripts should reach the cytoplasm for translation, eukaryotic cells
have established mechanisms to regulate selective mRNA export through the nuclear pore …

YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries

B Dubreuil, E Sass, Y Nadav… - Nucleic Acids …, 2019 - academic.oup.com
The ability to measure the abundance and visualize the localization of proteins across the
yeast proteome has stimulated hypotheses on gene function and fueled discoveries. While …

Membrane-anchored ubiquitin ligase complex is required for the turnover of lysosomal membrane proteins

M Li, T Koshi, SD Emr - Journal of Cell Biology, 2015 - rupress.org
Cells must regulate the abundance and activity of numerous nutrient transporters in different
organelle membranes to achieve nutrient homeostasis. As the recycling center and major …