In recent years, exceptional technological advances have enabled the identification and
interrogation of rare circulating tumour cells (CTCs) from blood samples of patients, leading …

Understanding intratumoral heterogeneity—the molecular variation among cells within a
tumor—promises to address outstanding questions in cancer biology and improve the …

We describe a workflow for preprocessing of single-cell RNA-sequencing data that balances
efficiency and accuracy. Our workflow is based on the kallisto and bustools programs, and is …

Heterogeneity within a tumour increases its ability to adapt to constantly changing
constraints, but adversely affects a patient's prognosis, therapy response and clinical …

Cancer cells enter a reversible drug-tolerant persister (DTP) state to evade death from
chemotherapy and targeted agents. It is increasingly appreciated that DTPs are important …

The success of targeted therapies and immunotherapies has created optimism that cancers
may be curable. However, not all patients respond, drug resistance is common and many …

Single-cell technologies have contributed to unravelling tumour heterogeneity, now
considered a hallmark of cancer and one of the main causes of tumour resistance to cancer …

Cancer relapse after chemotherapy remains a main cause of cancer-related death. Although
the relapse is thought to result from the propagation of resident cancer stem cells, a lack of …

The emergence of drug resistance is the most substantial challenge to the effectiveness of
anticancer therapies. Orthogonal approaches have revealed that a subset of cells, known as …

The analysis of circulating tumor cells (CTCs) is an outstanding tool to provide insights into
the biology of metastatic cancers, to monitor disease progression and with potential for use …