Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Deep-STORM: super-resolution single-molecule microscopy by deep learning

E Nehme, LE Weiss, T Michaeli, Y Shechtman - Optica, 2018 - opg.optica.org
We present an ultrafast, precise, parameter-free method, which we term Deep-STORM, for
obtaining super-resolution images from stochastically blinking emitters, such as fluorescent …

Stimulated emission depletion microscopy

H Blom, J Widengren - Chemical reviews, 2017 - ACS Publications
Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

[HTML][HTML] Breaking the diffraction barrier: super-resolution imaging of cells

B Huang, H Babcock, X Zhuang - Cell, 2010 - cell.com
Anyone who has used a light microscope has wished that its resolution could be a little
better. Now, after centuries of gradual improvements, fluorescence microscopy has made a …

Imaging intracellular fluorescent proteins at nanometer resolution

E Betzig, GH Patterson, R Sougrat, OW Lindwasser… - science, 2006 - science.org
We introduce a method for optically imaging intracellular proteins at nanometer spatial
resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules …

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

MJ Rust, M Bates, X Zhuang - Nature methods, 2006 - nature.com
We have developed a high-resolution fluorescence microscopy method based on high-
accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction …

Super-resolution fluorescence microscopy

B Huang, M Bates, X Zhuang - Annual review of biochemistry, 2009 - annualreviews.org
Achieving a spatial resolution that is not limited by the diffraction of light, recent
developments of super-resolution fluorescence microscopy techniques allow the …

Multicolor super-resolution imaging with photo-switchable fluorescent probes

M Bates, B Huang, GT Dempsey, X Zhuang - Science, 2007 - science.org
Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a
spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however …

High-resolution and large field-of-view Fourier ptychographic microscopy and its applications in biomedicine

A Pan, C Zuo, B Yao - Reports on Progress in Physics, 2020 - iopscience.iop.org
Fourier ptychographic microscopy (FPM) is a promising and fast-growing computational
imaging technique with high resolution, wide field-of-view (FOV) and quantitative phase …