Transfection is a powerful analytical tool enabling studies of gene products and functions in
eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity …

Approximately 75% of all disease-relevant human proteins, including those involved in
intracellular protein–protein interactions (PPIs), are undruggable with the current drug …

In today's modern era of medicine, macromolecular compounds such as proteins, peptides
and nucleic acids are dethroning small molecules as leading therapeutics. Given their …

The presence of cell and tissue barriers together with the low biomembrane permeability of
various therapeutics often hampers systemic drug distribution; thus, most of the available …

Introduction The biological membranes as natural permeable barriers are required for
survival and function of living cells. However, these natural barriers could be a major …

Protein structure underlies essential biological processes and provides a blueprint for
molecular mimicry that drives drug discovery. Although small molecules represent the lion's …

Enabling the cellular delivery and cytosolic bioavailability of functional proteins constitutes a
major challenge for the life sciences. Here we demonstrate that thiol-reactive arginine-rich …

Twenty years ago, the discovery of peptides able to cross cellular membranes launched a
novel field in molecular delivery based on these non-invasive vectors, most commonly …

The hydrophilic nature of peptides and proteins renders them impermeable to cell
membranes. Thus, in order to successfully deliver peptide and protein-based therapeutics …

Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid
membrane; they instead passively enter vesicles and live cells by inducing membrane …