Single-molecule localization microscopy
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
techniques that dramatically improve spatial resolution over standard, diffraction-limited …
Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects
S Zeng, X Liu, YS Kafuti, H Kim, J Wang… - Chemical Society …, 2023 - pubs.rsc.org
Since their inception, rhodamine dyes have been extensively applied in biotechnology as
fluorescent markers or for the detection of biomolecules owing to their good optical physical …
fluorescent markers or for the detection of biomolecules owing to their good optical physical …
Super-resolution microscopy demystified
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …
restricts the optical resolution to roughly 250 nm and was previously thought to be …
Imaging dynamic and selective low-complexity domain interactions that control gene transcription
INTRODUCTION DNA binding transcription factors (TFs) are quintessential regulators of
eukaryotic gene expression. Early studies of TFs revealed their well-structured DNA binding …
eukaryotic gene expression. Early studies of TFs revealed their well-structured DNA binding …
Spatially resolved mass spectrometry at the single cell: recent innovations in proteomics and metabolomics
Biological systems are composed of heterogeneous populations of cells that
intercommunicate to form a functional living tissue. Biological function varies greatly across …
intercommunicate to form a functional living tissue. Biological function varies greatly across …
RNA polymerase II clustering through carboxy-terminal domain phase separation
M Boehning, C Dugast-Darzacq, M Rankovic… - Nature structural & …, 2018 - nature.com
The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is an intrinsically disordered
low-complexity region that is critical for pre-mRNA transcription and processing. The CTD …
low-complexity region that is critical for pre-mRNA transcription and processing. The CTD …
Super-resolution microscopy for structural cell biology
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
Small-molecule fluorescent probes for live-cell super-resolution microscopy
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …
cellular structures at the nanometer scale. Employing these techniques in living cells has …
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Pushing the frontier of fluorescence microscopy requires the design of enhanced
fluorophores with finely tuned properties. We recently discovered that incorporation of four …
fluorophores with finely tuned properties. We recently discovered that incorporation of four …
Hybrid small-molecule/protein fluorescent probes
M Minoshima, SI Reja, R Hashimoto, K Iijima… - Chemical …, 2024 - ACS Publications
Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …
localization and function in living cells. These hybrid probes are constructed by diverse site …