Directed enzyme evolution has proven to be a powerful tool for improving a range of
properties of enzymes through consecutive rounds of diversification and selection. However …

In the last decade, directed evolution has become a routine approach for engineering
proteins with novel or altered properties. Concurrently, a trend away from purely …

This article describes the generation of the Human Combinatorial Antibody Library HuCAL
GOLD. HuCAL GOLD is a synthetic human Fab library based on the HuCAL concept with all …

Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet
their potential remains untapped in laboratory evolution. Here we introduce a transposon …

Small libraries for directed evolution can be obtained by neutral drifts that maintain the
protein's original function, yielding highly polymorphic, stable and evolvable variants. We …

One of the most vexing problems facing structural genomics efforts and the biotechnology
enterprise in general is the inability to efficiently produce functional proteins due to poor …

DNA synthesis techniques and technologies are quickly becoming a cornerstone of modern
molecular biology and play a pivotal role in the field of synthetic biology. The ability to …

Degenerate codon (DC) libraries efficiently address the experimental library-size limitations
of directed evolution by focusing diversity toward the positions and toward the amino acids …

Antibody phage display provides a powerful and efficient tool for the discovery and
development of monoclonal antibodies for therapeutic and other applications. Antibody …

In protein trans-splicing, an intein domain split into two polypeptide chains mediates linkage
of the flanking amino acid sequences, the N-and C-terminal exteins, with a native peptide …