The technological landscape and applications of single-cell multi-omics
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …
simultaneously integrating various single-modality omics methods that profile the …
Droplet-based microfluidics
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …
molecules have led to substantial improvements in accuracy, read length and throughput …
Single-cell long-read sequencing-based map** reveals specialized splicing patterns in develo** and adult mouse and human brain
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform
map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes …
map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes …
The great immune escape: understanding the divergent immune response in breast cancer subtypes
Breast cancer, the most common type of cancer affecting women, encompasses a collection
of histologic (mainly ductal and lobular) and molecular subtypes exhibiting diverse clinical …
of histologic (mainly ductal and lobular) and molecular subtypes exhibiting diverse clinical …
Spatial deconvolution of HER2-positive breast cancer delineates tumor-associated cell type interactions
In the past decades, transcriptomic studies have revolutionized cancer treatment and
diagnosis. However, tumor sequencing strategies typically result in loss of spatial …
diagnosis. However, tumor sequencing strategies typically result in loss of spatial …
High-throughput and single-cell T cell receptor sequencing technologies
T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
SQANTI3: curation of long-read transcriptomes for accurate identification of known and novel isoforms
SQANTI3 is a tool designed for the quality control, curation and annotation of long-read
transcript models obtained with third-generation sequencing technologies. Leveraging its …
transcript models obtained with third-generation sequencing technologies. Leveraging its …
Unraveling B cell trajectories at single cell resolution
During adaptive immunity, B cells differentiate either into memory B cells or plasma cells and
produce antibodies against foreign antigens to fight infection. Additionally, they behave as …
produce antibodies against foreign antigens to fight infection. Additionally, they behave as …
Spatial transcriptomics of B cell and T cell receptors reveals lymphocyte clonal dynamics
The spatial distribution of lymphocyte clones within tissues is critical to their development,
selection, and expansion. We have developed spatial transcriptomics of variable, diversity …
selection, and expansion. We have developed spatial transcriptomics of variable, diversity …