How to study proteins by circular dichroism

SM Kelly, TJ Jess, NC Price - Biochimica et Biophysica Acta (BBA)-Proteins …, 2005 - Elsevier
Circular dichroism (CD) is being increasingly recognised as a valuable technique for
examining the structure of proteins in solution. However, the value of many studies using CD …

Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases

L Whitmore, BA Wallace - Biopolymers: Original Research on …, 2008 - Wiley Online Library
Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein
secondary structures for many years. With the advent of synchrotron radiation circular …

DichroWeb, a website for calculating protein secondary structure from circular dichroism spectroscopic data

AJ Miles, SG Ramalli, BA Wallace - Protein Science, 2022 - Wiley Online Library
Circular dichroism (CD) spectroscopy is a widely‐used method for characterizing the
secondary structures of proteins. The well‐established and highly used analysis website …

BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra

A Micsonai, F Wien, É Bulyáki, J Kun… - Nucleic acids …, 2018 - academic.oup.com
Circular dichroism (CD) spectroscopy is a widely used method to study the protein
secondary structure. However, for decades, the general opinion was that the correct …

[HTML][HTML] The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis

J Li, T McQuade, AB Siemer, J Napetschnig… - Cell, 2012 - cell.com
RIP1 and RIP3 kinases are central players in TNF-induced programmed necrosis. Here, we
report that the RIP homotypic interaction motifs (RHIMs) of RIP1 and RIP3 mediate the …

G‐quadruplex secondary structure obtained from circular dichroism spectroscopy

R del Villar‐Guerra, JO Trent… - Angewandte Chemie, 2018 - Wiley Online Library
A curated library of circular dichroism spectra of 23 G‐quadruplexes of known structure was
built and analyzed. The goal of this study was to use this reference library to develop an …

ThT 101: a primer on the use of thioflavin T to investigate amyloid formation

K Gade Malmos, LM Blancas-Mejia, B Weber… - Amyloid, 2017 - Taylor & Francis
Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While
concerns have recently been raised about its use as a probe specific for amyloid, ThT still …

3D printed microneedle patches using stereolithography (SLA) for intradermal insulin delivery

SN Economidou, CPP Pere, A Reid, MJ Uddin… - Materials Science and …, 2019 - Elsevier
Abstract 3D printed microneedle arrays were fabricated using a biocompatible resin through
stereolithography (SLA) for transdermal insulin delivery. Microneedles were built by …

Evaluation of peptide/protein self-assembly and aggregation by spectroscopic methods

MF Pignataro, MG Herrera, VI Dodero - Molecules, 2020 - mdpi.com
The self-assembly of proteins is an essential process for a variety of cellular functions
including cell respiration, mobility and division. On the other hand, protein or peptide …

Extraction, physicochemical characteristics and functional properties of Mung bean protein

M Du, J **e, B Gong, X Xu, W Tang, X Li, C Li, M **e - Food hydrocolloids, 2018 - Elsevier
The physicochemical characteristics, functional properties and amino acid composition of
protein extracts from Mung bean were determined and analyzed for discovering its potential …