[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …
molecules have led to substantial improvements in accuracy, read length and throughput …
Overview of next‐generation sequencing technologies
BE Slatko, AF Gardner… - Current protocols in …, 2018 - Wiley Online Library
High throughput DNA sequencing methodology (next generation sequencing; NGS) has
rapidly evolved over the past 15 years and new methods are continually being …
rapidly evolved over the past 15 years and new methods are continually being …
Three decades of nanopore sequencing
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …
Nanopore-based fourth-generation DNA sequencing technology
Nanopore-based sequencers, as the fourth-generation DNA sequencing technology, have
the potential to quickly and reliably sequence the entire human genome for less than 1000 …
the potential to quickly and reliably sequence the entire human genome for less than 1000 …
Discrimination of oligonucleotides of different lengths with a wild-type aerolysin nanopore
Protein nanopores offer an inexpensive, label-free method of analysing single
oligonucleotides. The sensitivity of the approach is largely determined by the characteristics …
oligonucleotides. The sensitivity of the approach is largely determined by the characteristics …
[LIVRE][B] Solid-state nanopore sensors for nucleic acid analysis
BM Venkatesan, R Bashir - 2011 - Springer
Solid-state nanopores are nm sized apertures formed in thin synthetic membranes. These
single molecule sensors have been used in a variety of biophysical and diagnostic …
single molecule sensors have been used in a variety of biophysical and diagnostic …
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
Nanopore technologies are being developed for fast and direct sequencing of single DNA
molecules through detection of ionic current modulations as DNA passes through a pore's …
molecules through detection of ionic current modulations as DNA passes through a pore's …
The potential and challenges of nanopore sequencing
A nanopore-based device provides single-molecule detection and analytical capabilities
that are achieved by electrophoretically driving molecules in solution through a nano-scale …
that are achieved by electrophoretically driving molecules in solution through a nano-scale …
Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision
GM Cherf, KR Lieberman, H Rashid, CE Lam… - Nature …, 2012 - nature.com
An emerging DNA sequencing technique uses protein or solid-state pores to analyze
individual strands as they are driven in single-file order past a nanoscale sensor,,. However …
individual strands as they are driven in single-file order past a nanoscale sensor,,. However …
Controlling protein translocation through nanopores with bio-inspired fluid walls
Synthetic nanopores have been used to study individual biomolecules in high throughput,
but their performance as sensors does not match that of biological ion channels. Challenges …
but their performance as sensors does not match that of biological ion channels. Challenges …