The regulation of the translation of messenger RNA (mRNA) in eukaryotic cells is critical for
gene expression, and occurs principally at the initiation phase which is mainly regulated by …

Protein homeostasis of bacterial cells is maintained by coordinated processes of protein
production, folding, and degradation. Translational efficiency of a given mRNA depends on …

Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA
selection and translocation reactions that proceed rapidly (2–20 per second) and with a low …

Optimization of biological networks is often limited by wet lab labor and cost, and the lack of
convenient computational tools. Here, we describe METIS, a versatile active machine …

In the early stage of bacterial translation, peptidyl-tRNAs frequently dissociate from the
ribosome (pep-tRNA drop-off) and are recycled by peptidyl-tRNA hydrolase. Here, we …

Protein expression is a critical process in diverse biological systems. For Escherichia coli, a
widely employed microbial host in industrial catalysis and healthcare, researchers often face …

Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …

Translation efficiency has been mainly studied by ribosome profiling, which only provides an
incomplete picture of translation kinetics. Here, we integrated the absolute quantifications of …

Gene expression involves transcription and translation. Despite large datasets and
increasingly powerful methods devoted to calculating genetic variants' effects on …

Extremely diverse libraries are essential for effectively selecting functional peptides or
proteins, and mRNA display technology is a powerful tool for generating such libraries with …