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Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …
Perspectives and benefits of high-throughput long-read sequencing in microbial ecology
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …
insights into microbial ecology and function. Yet, in many instances short-read HTS …
Acute and persistent effects of commonly used antibiotics on the gut microbiome and resistome in healthy adults
Antibiotics are deployed against bacterial pathogens, but their targeting of conserved
microbial processes means they also collaterally perturb the commensal microbiome. To …
microbial processes means they also collaterally perturb the commensal microbiome. To …
Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection
The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …
Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
Adaptive sampling is a method of software-controlled enrichment unique to nanopore
sequencing platforms. To test its potential for enrichment of rarer species within …
sequencing platforms. To test its potential for enrichment of rarer species within …
Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution
Background Species-level genetic characterization of complex bacterial communities has
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …
Nanopore targeted sequencing for the accurate and comprehensive detection of SARS‐CoV‐2 and other respiratory viruses
M Wang, A Fu, B Hu, Y Tong, R Liu, Z Liu, J Gu… - Small, 2020 - Wiley Online Library
The ongoing global novel coronavirus pneumonia COVID‐19 outbreak has engendered
numerous cases of infection and death. COVID‐19 diagnosis relies upon nucleic acid …
numerous cases of infection and death. COVID‐19 diagnosis relies upon nucleic acid …
Maternal microbiota, early life colonization and breast milk drive immune development in the newborn
C Kalbermatter, N Fernandez Trigo… - Frontiers in …, 2021 - frontiersin.org
The innate immune system is the oldest protection strategy that is conserved across all
organisms. Although having an unspecific action, it is the first and fastest defense …
organisms. Although having an unspecific action, it is the first and fastest defense …
Hybrid metagenomic assembly enables high-resolution analysis of resistance determinants and mobile elements in human microbiomes
Abstract Characterization of microbiomes has been enabled by high-throughput
metagenomic sequencing. However, existing methods are not designed to combine reads …
metagenomic sequencing. However, existing methods are not designed to combine reads …
mNGS in clinical microbiology laboratories: on the road to maturity
Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …