[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …

Protist metabarcoding and environmental biomonitoring: time for change

J Pawlowski, F Lejzerowicz… - European Journal of …, 2016 - Elsevier
High-throughput amplicon sequencing of environmental DNA and/or RNA proved to be a
powerful tool to describe protist diversity. This new approach called also the metabarcoding …

Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data

NM Davis, DM Proctor, SP Holmes, DA Relman… - Microbiome, 2018 - Springer
Background The accuracy of microbial community surveys based on marker-gene and
metagenomic sequencing (MGS) suffers from the presence of contaminants—DNA …

CRIS. py: a versatile and high-throughput analysis program for CRISPR-based genome editing

JP Connelly, SM Pruett-Miller - Scientific reports, 2019 - nature.com
CRISPR-Cas9 technology allows the creation of user-defined genomic modifications in cells
and whole organisms. However, quantifying editing rates in pools of cells or identifying …

Short-term nitrogen fertilization affects microbial community composition and nitrogen mineralization functions in an agricultural soil

Y Ouyang, JM Norton - Applied and Environmental Microbiology, 2020 - Am Soc Microbiol
Soil extracellular enzymes play a significant role in the N mineralization process. However,
few studies have documented the linkage between enzyme activity and the microbial …

Diazotroph Community Characterization via a High-Throughput nifH Amplicon Sequencing and Analysis Pipeline

JC Gaby, L Rishishwar… - Applied and …, 2018 - Am Soc Microbiol
The dinitrogenase reductase gene (nifH) is the most widely established molecular marker for
the study of nitrogen-fixing prokaryotes in nature. A large number of PCR primer sets have …

Distribution of mixotrophy and desiccation survival mechanisms across microbial genomes in an arid biological soil crust community

DV Meier, S Imminger, O Gillor, D Woebken - Msystems, 2021 - Am Soc Microbiol
Desert surface soils devoid of plant cover are populated by a variety of microorganisms,
many with yet unresolved physiologies and lifestyles. Nevertheless, a common feature vital …

Impacts of arsenic and antimony co-contamination on sedimentary microbial communities in rivers with different pollution gradients

X Sun, B Li, F Han, E **ao, T **ao, W Sun - Microbial ecology, 2019 - Springer
Abstract Arsenic (As) and antimony (Sb) are both toxic metalloids that are of primary concern
for human health. Mining activity has introduced elevated levels of arsenic and antimony into …

Impact of peat mining and restoration on methane turnover potential and methane-cycling microorganisms in a northern bog

M Reumer, M Harnisz, HJ Lee, A Reim… - Applied and …, 2018 - Am Soc Microbiol
Ombrotrophic peatlands are a recognized global carbon reservoir. Without restoration and
peat regrowth, harvested peatlands are dramatically altered, impairing their carbon sink …

Crop diversification improves the diversity and network structure of the prokaryotic soil microbiome at conventional nitrogen fertilization

B Liu, J Schroeder, H Ahnemann, C Poeplau… - Plant and Soil, 2023 - Springer
Background Sustainable agriculture seeks to optimize the application of nitrogen (N)
fertilizers to reduce adverse economic and ecological effects. Crop diversification has been …