Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Microorganisms produce a wealth of structurally diverse specialized metabolites with a
remarkable range of biological activities and a wide variety of applications in medicine and …

High-sugar diet causes health problems, many of which can be addressed with the use of
sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered the …

The industrial yeast Pichia pastoris has been harnessed extensively for production of
proteins, and it is attracting attention as a chassis cell factory for production of chemicals …

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …

The tetrahydroisoquinoline (THIQ) moiety is a privileged substructure of many bioactive
natural products and semi-synthetic analogs. Plants manufacture more than 3,000 THIQ …

Actinobacteria, particularly those of genus Streptomyces, remain invaluable hosts for the
discovery and engineering of natural products and their cognate biosynthetic pathways …

Recombinant protein production emerged in the early 1980s with the development of
genetic engineering tools, which represented a compelling alternative to protein extraction …

Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …