Drug discovery targeting amino acid racemases

P Conti, L Tamborini, A Pinto, A Blondel… - Chemical …, 2011 - ACS Publications
Amino acids are among the most important molecules in nature since they play central roles
both as building blocks of proteins and as intermediates in metabolism. The amino acid …

The coordinated action of the enzymes in the L-lysine biosynthetic pathway and how to inhibit it for antibiotic targets

S Muduli, S Karmakar, S Mishra - Biochimica et Biophysica Acta (BBA) …, 2023 - Elsevier
Background Antimicrobial resistance is a global health issue that requires immediate
attention in terms of new antibiotics and new antibiotic targets. The l-lysine biosynthesis …

[HTML][HTML] Structural snapshots of nitrosoglutathione binding and reactivity underlying S-nitrosylation of photosynthetic GAPDH

EJ Mattioli, J Rossi, M Meloni, M De Mia, CH Marchand… - Redox Biology, 2022 - Elsevier
S-nitrosylation is a redox post-translational modification widely recognized to play an
important role in cellular signaling as it can modulate protein function and conformation. At …

Tuning Cysteine Reactivity and Sulfenic Acid Stability by Protein Microenvironment in Glyceraldehyde-3-Phosphate Dehydrogenases of Arabidopsis thaliana

M Zaffagnini, S Fermani, M Calvaresi… - Antioxidants & Redox …, 2016 - liebertpub.com
Abstract Aims: Cysteines and H2O2 are fundamental players in redox signaling. Cysteine
thiol deprotonation favors the reaction with H2O2 that generates sulfenic acids with dual …

Structural basis for redox sensitivity in Corynebacterium glutamicum diaminopimelate epimerase: an enzyme involved in l-lysine biosynthesis

HY Sagong, KJ Kim - Scientific reports, 2017 - nature.com
Diaminopimelate epimerase (DapF) is one of the crucial enzymes involved in l-lysine
biosynthesis, where it converts l, l-diaminopimelate (l, l-DAP) into d, l-DAP. DapF is also …

Conformational Dynamics in Corynebacterium glutamicum Diaminopimelate Epimerase: Insights from Ligand Parameterization, Atomistic Simulation, and Markov …

S Muduli, S Karmakar, S Mishra - Journal of Chemical Information …, 2024 - ACS Publications
The microbial enzyme diaminopimelate epimerase (DapF), a vital enzyme in the lysine
biosynthetic pathway, catalyzes the conversion of l, l-diaminopimelate (l, l-DAP) to d, l …

Carbon acidity in enzyme active sites

MD Toney - Frontiers in Bioengineering and Biotechnology, 2019 - frontiersin.org
The pKa values for substrates acting as carbon acids (ie, CH deprotonation reactions) in
several enzyme active sites are presented. The information needed to calculate them …

Insight into the binding interactions of CYP450 aromatase inhibitors with their target enzyme: a combined molecular docking and molecular dynamics study

R Galeazzi, L Massaccesi - Journal of molecular modeling, 2012 - Springer
CYP450 aromatase catalyzes the terminal and rate-determining step in estrogen synthesis,
the aromatization of androgens, and its inhibition is an efficient approach to treating …

Characterization of DcsC, a PLP-independent racemase involved in the biosynthesis of D-cycloserine

D Dietrich, MJ van Belkum, JC Vederas - Organic & Biomolecular …, 2012 - pubs.rsc.org
The biosynthetic gene cluster responsible for the generation of the antibiotic D-cycloserine
(DCS) has recently been disclosed. One of the putative enzymes described was DcsC …

Computational evidence for the catalytic mechanism of tyrosylprotein sulfotransferases: a density functional theory investigation

TD Marforio, P Giacinto, A Bottoni, M Calvaresi - Biochemistry, 2015 - ACS Publications
In this paper we have examined the mechanism of tyrosine O-sulfonation catalyzed by
human TPST-2. Our computations, in agreement with Teramoto's hypothesis, indicate a …