This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekee** genes …

Given the highly dynamic nature of mRNA transcription and the potential variables
introduced in sample handling and in the downstream processing steps (Garson et …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a
commercially viable source of biomass for bioelectricity and second-generation bioethanol …

The development of shoot-borne roots, or adventitious roots, is indispensable for mass
propagation of elite genotypes. It is a complex genetic trait with a high phenotypic plasticity …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Leaf aging is a highly regulated developmental process, which is also influenced profoundly
by diverse environmental conditions. Accumulating evidence in recent years supports that …