Highlights•A public–private partnership targets drug-binding kinetics in a multilevel
approach.•New experimental approaches for measuring drug residence time are …

The structure of a protein–protein interaction, its affinity and thermodynamic characteristics
depict a 'frozen'state of a complex. This picture ignores the kinetic nature of complex …

The interaction of biologicalmacromolecules, whether protein–DNA, antibody–antigen,
hormone–receptor, etc., illustrates the complexity and diversity of molecular recognition. The …

In this issue of the Proceedings, Roost et al.(1) describe the development of humoral
immunity in mice infected with vesicular stomatitis virus (VSV). The authors sac-rificed mice …

Intrinsically disordered proteins (IDPs) are recognized to play important roles in many
biological functions such as transcription and translation regulation, cellular signal …

Induced fit explains why biomolecules can bind together even if they are not optimized for
binding. However, induced fit can lead to a kinetic bottleneck and does not describe every …

The rate of protein-protein association limits the response time due to protein-protein
interactions. The bimolecular association rate may be diffusion-controlled or influenced, and …

Tetraspanins, including CD9, CD63, and CD81, are transmembrane biomarkers that play a
crucial role in regulating cancer cell proliferation, invasion, and metastasis, as well as …

β2-Glycoprotein I (β2GPI) is an essential cofactor for the binding to lipids of anticardiolipin
antibodies (ACA), isolated from patients with anti-phospholipid syndrome. We used …

Complexes between Src-homology 3 domains and proline-rich target peptides can have
lifetimes on the order of milliseconds, making them too short-lived for kinetic characterization …