Mycobiome diversity: high-throughput sequencing and identification of fungi

RH Nilsson, S Anslan, M Bahram… - Nature Reviews …, 2019 - nature.com
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …

Fungal identification using molecular tools: a primer for the natural products research community

HA Raja, AN Miller, CJ Pearce… - Journal of natural …, 2017 - ACS Publications
Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They
are known to produce numerous bioactive molecules, which makes them very useful for …

Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin

NA Bokulich, BD Kaehler, JR Rideout, M Dillon… - Microbiome, 2018 - Springer
Background Taxonomic classification of marker-gene sequences is an important step in
microbiome analysis. Results We present q2-feature-classifier (https://github. com/qiime2/q2 …

RESCRIPt: Reproducible sequence taxonomy reference database management

MS Robeson, DR O'Rourke, BD Kaehler… - PLoS computational …, 2021 - journals.plos.org
Nucleotide sequence and taxonomy reference databases are critical resources for
widespread applications including marker-gene and metagenome sequencing for …

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

R Lücking, MC Aime, B Robbertse, AN Miller… - IMA fungus, 2020 - Springer
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …

[HTML][HTML] Microbial interkingdom interactions in roots promote Arabidopsis survival

P Durán, T Thiergart, R Garrido-Oter, M Agler, E Kemen… - Cell, 2018 - cell.com
Roots of healthy plants are inhabited by soil-derived bacteria, fungi, and oomycetes that
have evolved independently in distinct kingdoms of life. How these microorganisms interact …

Ecological memory of recurrent drought modifies soil processes via changes in soil microbial community

A Canarini, H Schmidt, L Fuchslueger, V Martin… - Nature …, 2021 - nature.com
Climate change is altering the frequency and severity of drought events. Recent evidence
indicates that drought may produce legacy effects on soil microbial communities. However, it …

SINTAX: a simple non-Bayesian taxonomy classifier for 16S and ITS sequences

RC Edgar - biorxiv, 2016 - biorxiv.org
Metagenomics experiments often characterize microbial communities by sequencing the
ribosomal 16S and ITS regions. Taxonomy prediction is a fundamental step in such studies …

Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

IDTAXA: a novel approach for accurate taxonomic classification of microbiome sequences

A Murali, A Bhargava, ES Wright - Microbiome, 2018 - Springer
Background Microbiome studies often involve sequencing a marker gene to identify the
microorganisms in samples of interest. Sequence classification is a critical component of this …