Here we describe YESS 2.0, a highly versatile version of the yeast endoplasmic
sequestration screening (YESS) system suitable for engineering and characterizing …

An enzyme engineering technology involving yeast endoplasmic reticulum (ER)
sequestration screening (YESS) has been recently developed. Here, a new method is …

An endoplasmic reticulum (ER) retention sequence (ERS) is a characteristic short sequence
that mediates protein retention in the ER of eukaryotic cells. However, little is known about …

Human rhinovirus 3C protease (HRV 3C-P) is a high-value commercial cysteine protease
that could specifically recognize the short peptide sequence of LEVLFQ↓ GP. In here, a …

Cells sense cues in their surrounding environment, turning extracellular stimuli into
intracellular responses. Transient posttranslational modifications, such as phosphorylation …

For antibody discovery and engineering, yeast surface display (YSD) of antigen-binding
fragments (Fabs) and coupled fluorescence activated cell sorting (FACS) provide intact …

Human rhinovirus 3C protease (HRV 3Csingle bondP) has a high specificity against the
substrate of LEVLFQ↓ G at P1′ site, which plays an important role in biotechnology and …

Characterizing the substrate specificity of protease enzymes is critical for illuminating the
molecular basis of their diverse and complex roles in a wide array of biological processes …

Efficient identification of epitopes is crucial for drug discovery and design as it enables the
selection of optimal epitopes, expansion of lead antibody diversity, and verification of …

The ability to understand and characterize phosphorylation is important to the study of cell
signaling and to synthetic biology approaches. Current methods for characterizing kinase …