Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …

Different species of microorganisms including yeasts, filamentous fungi and bacteria have
been used in the past 25 years for the controlled production of foreign proteins of scientific …

Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant
bacteria showing analogies with secretory granules of the mammalian endocrine system …

The temperature inducible expression system, based on the pL and/or pR phage lambda
promoters regulated by the thermolabile cI857 repressor has been widely use to produce …

Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Increasing evidence indicates that protein aggregation in bacteria does not necessarily
imply loss of biological activity. Here, we have investigated the effect of growth-temperature …

The formation of inclusion bodies (IBs) constitute a frequent event during the production of
heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation …

Insufficient availability of molecular chaperones is observed as a major bottleneck for proper
protein folding in recombinant protein production. Therefore, co-production of selected sets …

Protein production under the control of lac operon regulatory elements using autoinduction
is based on diauxic growth of Escherichia coli on lactose after consumption of more …

Despite the fundamental importance of E. coli in the manufacture of a wide range of
biotechnological and biomedical products, extensive process and/or target optimisation is …