Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this,
many of these receptors have limited or no available prophylaxis. Novel drug design and …

Over the last years protein engineering using non-standard amino acids has gained
increasing attention. As a result, improved methods are now available, enabling the efficient …

About ten years ago the characterization of an uncultivated marine bacterium revealed a
new type of retinal-binding, integral membrane protein called proteorhodopsin (PR).[1] Many …

Membrane proteins frequently assemble into higher order homo-or hetero-oligomers within
their natural lipid environment. This complex formation can modulate their folding, activity as …

Neutron scattering studies provide important information in structural biology that is not
accessible using other approaches. The uniqueness of the technique, and its …

Cell-free expression has emerged as a powerful technique to overcome major restrictions of
classical in vivo membrane protein production, with sample yields of mgms of protein per ml …

NMR structural studies of membrane proteins (MP) are hampered by complications in MP
expression, technical difficulties associated with the slow process of NMR spectral peak …

Many biological functions of peptides are mediated through G protein-coupled receptors
(GPCRs). Upon ligand binding, GPCRs undergo conformational changes that facilitate the …

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality
solution and solid-state NMR spectra of biological samples, with emphasis on integral …

Integral membrane proteins (IMPs) perform unique and indispensable functions in the cell,
making them attractive targets for fundamental research and drug discovery. Developments …