Clarifying tissue clearing

DS Richardson, JW Lichtman - Cell, 2015‏ - cell.com
Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …

Three-photon neuronal imaging in deep mouse brain

T Wang, C Xu - Optica, 2020‏ - opg.optica.org
Three-photon microscopy has been increasingly adopted for probing neural activities
beyond the typical two-photon imaging depth. In this review, we outline the unique …

A platform for brain-wide imaging and reconstruction of individual neurons

MN Economo, NG Clack, LD Lavis, CR Gerfen… - elife, 2016‏ - elifesciences.org
The structure of axonal arbors controls how signals from individual neurons are routed
within the mammalian brain. However, the arbors of very few long-range projection neurons …

Multiphoton fluorescence microscopy for in vivo imaging

C Xu, M Nedergaard, DJ Fowell, P Friedl, N Ji - Cell, 2024‏ - cell.com
Multiphoton fluorescence microscopy (MPFM) has been a game-changer for optical
imaging, particularly for studying biological tissues deep within living organisms. MPFM …

Light microscopy of proteins in their ultrastructural context

O M'Saad, J Bewersdorf - Nature communications, 2020‏ - nature.com
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain

T Wang, C Wu, DG Ouzounov, W Gu, F **a, M Kim… - Elife, 2020‏ - elifesciences.org
1300 nm three-photon calcium imaging has emerged as a useful technique to allow calcium
imaging in deep brain regions. Application to large-scale neural activity imaging entails a …

Morphogenesis guided by 3D patterning of growth factors in biological matrices

N Broguiere, I Lüchtefeld, L Trachsel… - Advanced …, 2020‏ - Wiley Online Library
Abstract Three‐dimensional (3D) control over the placement of bioactive cues is
fundamental to understand cell guidance and develop engineered tissues. Two‐photon …

[HTML][HTML] Fundamentals of light-cell–polymer interactions in photo-cross-linking based bioprinting

D Nieto, JA Marchal Corrales, A Jorge de Mora… - APL …, 2020‏ - pubs.aip.org
Biofabrication technologies that use light for polymerization of biomaterials have made
significant progress in the quality, resolution, and generation of precise complex tissue …

Large-scale automated histology in the pursuit of connectomes

D Kleinfeld, A Bharioke, P Blinder, DD Bock… - Journal of …, 2011‏ - jneurosci.org
How does the brain compute? Answering this question necessitates neuronal connectomes,
annotated graphs of all synaptic connections within defined brain areas. Further …

Temporally focused femtosecond laser pulses for low numerical aperture micromachining through optically transparent materials

DN Vitek, DE Adams, A Johnson, PS Tsai, S Backus… - Optics express, 2010‏ - opg.optica.org
Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous
limitations for ablating high aspect ratio features with low numerical aperture (NA) beams …