Comprehensively charting the biologically causal circuits that govern the phenotypic space
of human cells has often been viewed as an insurmountable challenge. However, in the last …

The ability to rationally engineer microorganisms has been a long-envisioned goal dating
back more than a half-century. With the genomics revolution and rise of systems biology in …

Abstract RegulonDB (http://regulondb. ccg. unam. mx) is one of the most useful and
important resources on bacterial gene regulation, as it integrates the scattered scientific …

Adaptive laboratory evolution is a frequent method in biological studies to gain insights into
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …

To exist in a wide range of environmental niches, bacteria must sense and respond to a
variety of external signals. A primary means by which this occurs is through two-component …

Transcription factors are key cellular components that control gene expression: their
activities determine how cells function and respond to the environment. Currently, there is …

The engineering of genetic circuits with predictive functionality in living cells represents a
defining focus of the expanding field of synthetic biology. This focus was elegantly set in …

Genome‐modification technologies enable the rational engineering and perturbation of
biological systems. Historically, these methods have been limited to gene insertions or …

Network inference, which is the reconstruction of biological networks from high-throughput
data, can provide valuable information about the regulation of gene expression in cells …

Single-cell measurements and lineage-tracing experiments are revealing that phenotypic
cell-to-cell variability is often the result of deterministic processes, despite the existence of …