Reagents that bind tightly and specifically to biomolecules of interest remain essential in the
exploration of biology and in their ultimate application to medicine. Besides ligands for …

ABSTRACT A milestone in the field of recombinant binding molecules was achieved 30
years ago with the discovery of single-domain antibodies from which antigen-binding …

Introduction Monoclonal antibodies have revolutionized personalized medicine for cancer in
recent decades. Despite their broad application in oncology, their large size and complexity …

Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology,
polarity and movement. Therefore, probing Rho GTPase activity is key to understanding …

The identification of diagnostic and therapeutic targets requires a comprehensive
understanding of cellular processes, for which advanced technologies in biomedical …

Protein–protein interactions (PPIs) form the foundation of any cell signaling network.
Considering that PPIs are highly dynamic processes, cellular assays are often essential for …

Sera of camelid species contain a special kind of antibody that consists only of heavy
chains. The variable antigen binding domain of these heavy chain antibodies can be …

Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the
past five to ten years, many new labelling tools have been developed with significant …

Simple Summary γ-H2AX, a phosphorylated variant of histone H2A, is a widely used
biomarker of DNA replication stress. To develop an immunological probe able to detect and …

Single-domain antibody fragments, also known as VHHs or nanobodies, have opened
promising avenues in therapeutics and in exploration of intracellular processes. Because of …