Protein engineering of Bacillus acidopullulyticus pullulanase for enhanced thermostability using in silico data driven rational design methods

A Chen, Y Li, J Nie, B McNeil, L Jeffrey, Y Yang… - Enzyme and microbial …, 2015 - Elsevier
Thermostability has been considered as a requirement in the starch processing industry to
maintain high catalytic activity of pullulanase under high temperatures. Four data driven …

Improving alkaline stability of α-L-rhamnosidase from Aspergillus Niger through computational strategy combines with folding free energy and binding free energy

L Li, C Peng, J Gong, X Liu, W Li, Y Zhu, H Ni… - Biochemical Engineering …, 2023 - Elsevier
It is difficult to improve pH stability of enzymes. In the present study, a semi-rational strategy
was validated to improve the alkaline stability of an α-L-rhamnosidase. The strategy …

Identification and Structural Analysis of Amino Acid Substitutions that Increase the Stability and Activity of Aspergillus niger Glucose Oxidase

J Marín-Navarro, N Roupain, D Talens-Perales… - PLoS …, 2015 - journals.plos.org
Glucose oxidase is one of the most conspicuous commercial enzymes due to its many
different applications in diverse industries such as food, chemical, energy and textile. Among …

Role of His-His interaction in Ser474-His475-Tyr476 sequence of chondroitinase ABC I in the enzyme activity and stability

M Maleki, K Khajeh, M Amanlou, A Golestani - International Journal of …, 2018 - Elsevier
Despite clinical importance of chondroitinase ABC I, its application has been limited due to
thermal instability as reported in the literature. There are various approaches to improve …

Establishment of aromatic pairs at the surface of chondroitinase ABC I: the effect on activity and stability

ME Shahaboddin, K Khajeh, A Golestani - Applied Biochemistry and …, 2018 - Springer
Removal of chondroitin sulfate glycosaminoglycan (GAG) chains with chondroitinase ABC I
(chABC I) in CNS injury models promotes both saxon regeneration and plasticity. It has been …

Methylglyoxal and high glucose inhibit VEGFR2 phosphorylation at specific tyrosine residues

F Betting, G Schlunck, HT Agostini… - … für Naturforschung C, 2022 - degruyter.com
Diabetes is characterized by hyperglycemia and a significant risk of vascular complications.
Vascular endothelial growth factor (VEGF) and its main receptor VEGFR2 (KDR), which is …

Engineering Saccharomyces cerevisiae for the production of 1, 2-propanediol using glycerol as a carbon and energy source

Z Islam - 2017 - opus.constructor.university
Biodiesel production via transesterification generates approx. 10%(w/w) glycerol, which is
considered as a glut and needs to be valorized. The production of 1, 2-propanediol (1, 2 …