[HTML][HTML] Mechanisms and regulation of replication fork reversal

MB Adolph, D Cortez - DNA repair, 2024 - Elsevier
DNA replication is remarkably accurate with estimates of only a handful of mutations per
human genome per cell division cycle. Replication stress caused by DNA lesions …

Control of DNA replication in vitro using a reversible replication barrier

EJ Vontalge, T Kavlashvili, SN Dahmen, MT Cranford… - Nature protocols, 2024 - nature.com
A major obstacle to studying DNA replication is that it involves asynchronous and highly
delocalized events. A reversible replication barrier overcomes this limitation and allows …

[HTML][HTML] BRCA2 promotes genomic integrity and therapy resistance primarily through its role in homology-directed repair

PX Lim, M Zaman, W Feng, M Jasin - Molecular cell, 2024 - cell.com
Tumor suppressor BRCA2 functions in homology-directed repair (HDR), the protection of
stalled replication forks, and the suppression of replicative gaps, but their relative …

Structural basis for stabilisation of the RAD51 nucleoprotein filament by BRCA2

R Appleby, L Joudeh, K Cobbett, L Pellegrini - Nature Communications, 2023 - nature.com
The BRCA2 tumour suppressor protein preserves genomic integrity via interactions with the
DNA-strand exchange RAD51 protein in homology-directed repair. The RAD51-binding TR2 …

Poly (ADP-ribosyl) ation of TIMELESS limits DNA replication stress and promotes stalled fork protection

J Rageul, N Lo, AL Phi, JA Patel, JJ Park, H Kim - Cell reports, 2024 - cell.com
Summary Poly (ADP-ribosyl) ation (PARylation), catalyzed mainly by poly (ADP-ribose)
polymerase (PARP) 1, is a key posttranslational modification involved in DNA replication …

Loo** out of control: R-loops in transcription-replication conflict

C Kumar, D Remus - Chromosoma, 2024 - Springer
Transcription-replication conflict is a major cause of replication stress that arises when
replication forks collide with the transcription machinery. Replication fork stalling at sites of …

Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair

E Colicino-Murbach, C Hathaway… - Nucleic Acids …, 2024 - academic.oup.com
Eukaryotic chromosomal replication occurs in a segmented, temporal manner wherein open
euchromatin and compact heterochromatin replicate during early and late S-phase …

Synergistic protection of nascent DNA at stalled forks by MSANTD4 and BRCA1/2–RAD51

H **e, L Song, G Mao, J Han, J Pu, Z Wu… - Nature Chemical …, 2025 - nature.com
The regressed arms of reversed replication forks exhibit structural similarities to one-ended
double-stranded breaks and need to be protected against uncontrolled nucleolytic …

Disparate requirements for RAD54L in replication fork reversal

ME Uhrig, N Sharma, P Maxwell, J Gomez… - Nucleic acids …, 2024 - academic.oup.com
Abstract RAD54L is a DNA motor protein with multiple roles in homologous recombination
DNA repair. In vitro, RAD54L was shown to also catalyze the reversal and restoration of …

[HTML][HTML] RTEL1 and MCM10 overcome topological stress during vertebrate replication termination

LV Campos, SX Van Ravenstein, EJ Vontalge… - Cell reports, 2023 - cell.com
Topological stress can cause converging replication forks to stall during termination of
vertebrate DNA synthesis. However, replication forks ultimately overcome fork stalling …