Inhibitors of bacterial immune systems: discovery, mechanisms and applications
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
Past, present, and future of CRISPR genome editing technologies
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …
offering unprecedented opportunities to dissect complex biological processes and treat the …
Biology and applications of CRISPR–Cas12 and transposon-associated homologs
CRISPR-associated Cas12 proteins are a highly variable collection of nucleic acid-targeting
proteins. All Cas12 variants use RNA guides and a single nuclease domain to target …
proteins. All Cas12 variants use RNA guides and a single nuclease domain to target …
Structural variation of types IV-A1-and IV-A3-mediated CRISPR interference
R Čepaitė, N Klein, A Mikšys, S Camara-Wilpert… - Nature …, 2024 - nature.com
CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and
nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge …
nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge …
Structural basis for the activity of the type VII CRISPR–Cas system
J Yang, X Li, Q He, X Wang, J Tang, T Wang, Y Zhang… - Nature, 2024 - nature.com
The newly identified type VII CRISPR–Cas candidate system uses a CRISPR RNA-guided
ribonucleoprotein complex formed by Cas5 and Cas7 proteins to target RNA. However, the …
ribonucleoprotein complex formed by Cas5 and Cas7 proteins to target RNA. However, the …
Discovering CRISPR-Cas system with self-processing pre-crRNA capability by foundation models
W Li, X Jiang, W Wang, L Hou, R Cai, Y Li, Q Gu… - Nature …, 2024 - nature.com
The discovery of CRISPR-Cas systems has paved the way for advanced gene editing tools.
However, traditional Cas discovery methods relying on sequence similarity may miss distant …
However, traditional Cas discovery methods relying on sequence similarity may miss distant …
Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics
Y Zhu, M Zhang, Z Jie, S Guo, Z Zhu, S Tao - Veterinary Research, 2024 - Springer
African swine fever (ASF) is a devastating disease caused by African swine fever virus
(ASFV) and leads to significant economic losses in the pig farming industry. Given the …
(ASFV) and leads to significant economic losses in the pig farming industry. Given the …
Rapid and sensitive protein complex alignment with foldseek-multimer
Advances in computational structure prediction will vastly augment the hundreds of
thousands of currently-available protein complex structures. Translating these into …
thousands of currently-available protein complex structures. Translating these into …
Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells
J Guo, L Gong, H Yu, M Li, Q An, Z Liu, S Fan… - Nature …, 2024 - nature.com
Abstract Type I CRISPR-Cas systems are widespread and have exhibited high versatility
and efficiency in genome editing and gene regulation in prokaryotes. However, due to the …
and efficiency in genome editing and gene regulation in prokaryotes. However, due to the …
CRISPR in mobile genetic elements: counter-defense, inter-element competition and RNA-guided transposition
CRISPR are adaptive immunity systems that protect bacteria and archaea from viruses and
other mobile genetic elements (MGE) via an RNA-guided interference mechanism …
other mobile genetic elements (MGE) via an RNA-guided interference mechanism …