Principles governing the phase separation of multidomain proteins

P Mohanty, U Kapoor… - Biochemistry, 2022 - ACS Publications
A variety of membraneless organelles, often termed “biological condensates”, play an
important role in the regulation of cellular processes such as gene transcription, translation …

Conformational ensembles of intrinsically disordered proteins and flexible multidomain proteins

FE Thomasen, K Lindorff-Larsen - Biochemical Society …, 2022 - portlandpress.com
Intrinsically disordered proteins (IDPs) and multidomain proteins with flexible linkers show a
high level of structural heterogeneity and are best described by ensembles consisting of …

Physics-driven coarse-grained model for biomolecular phase separation with near-quantitative accuracy

JA Joseph, A Reinhardt, A Aguirre, PY Chew… - Nature Computational …, 2021 - nature.com
Various physics-and data-driven sequence-dependent protein coarse-grained models have
been developed to study biomolecular phase separation and elucidate the dominant …

Accurate model of liquid–liquid phase behavior of intrinsically disordered proteins from optimization of single-chain properties

G Tesei, TK Schulze, R Crehuet… - Proceedings of the …, 2021 - National Acad Sciences
Many intrinsically disordered proteins (IDPs) may undergo liquid–liquid phase separation
(LLPS) and participate in the formation of membraneless organelles in the cell, thereby …

A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain

P Mohanty, J Shenoy, A Rizuan… - Proceedings of the …, 2023 - National Acad Sciences
TAR DNA-binding protein 43 (TDP-43) is involved in key processes in RNA metabolism and
is frequently implicated in many neurodegenerative diseases, including amyotrophic lateral …

Improved predictions of phase behaviour of intrinsically disordered proteins by tuning the interaction range

G Tesei, K Lindorff-Larsen - Open Research Europe, 2023 - pmc.ncbi.nlm.nih.gov
The formation and viscoelastic properties of condensates of intrinsically disordered proteins
(IDPs) is dictated by amino acid sequence and solution conditions. Because of the …

Expanding the molecular language of protein liquid–liquid phase separation

S Rekhi, CG Garcia, M Barai, A Rizuan, BS Schuster… - Nature Chemistry, 2024 - nature.com
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …

Sequence-dependent material properties of biomolecular condensates and their relation to dilute phase conformations

D Sundaravadivelu Devarajan, J Wang… - Nature …, 2024 - nature.com
Material properties of phase-separated biomolecular condensates, enriched with disordered
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …

Design of intrinsically disordered protein variants with diverse structural properties

F Pesce, A Bremer, G Tesei, JB Hopkins, CR Grace… - Science …, 2024 - science.org
Intrinsically disordered proteins (IDPs) perform a broad range of functions in biology,
suggesting that the ability to design IDPs could help expand the repertoire of proteins with …

Direct prediction of intrinsically disordered protein conformational properties from sequence

JM Lotthammer, GM Ginell, D Griffith, R Emenecker… - Biophysical …, 2024 - cell.com
Intrinsically disordered regions (IDRs) are ubiquitous across all domains of life and play a
range of functional roles. While folded domains are generally well-described by a stable 3D …