Advancing CRISPR/Cas gene editing with machine learning
JHC Fong, ASL Wong - Current Opinion in Biomedical Engineering, 2023 - Elsevier
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) system is a powerful tool for gene editing. Recent advancement and adaptation of …
(Cas) system is a powerful tool for gene editing. Recent advancement and adaptation of …
A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies
The success of CRISPR-mediated gene perturbation studies is highly dependent on the
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
Epigenetic profiles guide improved CRISPR/Cas9-mediated gene knockout in human T cells
Y Ito, S Inoue, T Nakashima, H Zhang, Y Li… - Nucleic acids …, 2024 - academic.oup.com
Genetic modification of specific genes is emerging as a useful tool to enhance the functions
of antitumor T cells in adoptive immunotherapy. Current advances in CRISPR/Cas9 …
of antitumor T cells in adoptive immunotherapy. Current advances in CRISPR/Cas9 …
Modular vector assembly enables rapid assessment of emerging CRISPR technologies
The diversity of CRISPR systems, coupled with scientific ingenuity, has led to an explosion
of applications; however, to test newly described innovations in their model systems …
of applications; however, to test newly described innovations in their model systems …
AI-Based solutions for current challenges in regenerative medicine
Abstract The emergence of Artificial Intelligence (AI) and its usage in regenerative medicine
represents a significant opportunity that holds the promise of tackling critical challenges and …
represents a significant opportunity that holds the promise of tackling critical challenges and …
A STAG2-PAXIP1/PAGR1 axis suppresses lung tumorigenesis
The cohesin complex is a critical regulator of gene expression. STAG2 is the most frequently
mutated cohesin subunit across several cancer types and is a key tumor suppressor in lung …
mutated cohesin subunit across several cancer types and is a key tumor suppressor in lung …
Guide-specific loss of efficiency and off-target reduction with Cas9 variants
High-fidelity clustered regularly interspaced palindromic repeats (CRISPR)-associated
protein 9 (Cas9) variants have been developed to reduce the off-target effects of CRISPR …
protein 9 (Cas9) variants have been developed to reduce the off-target effects of CRISPR …
[HTML][HTML] Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC)
CRISPR screens enable systematic, scalable genotype-to-phenotype map**. We
previously developed a pooled CRISPR screening method for Drosophila melanogaster and …
previously developed a pooled CRISPR screening method for Drosophila melanogaster and …
Higher-order combinatorial chromatin perturbations by engineered CRISPR-Cas12a for functional genomics
Multiplexed genetic perturbations are critical for testing functional interactions among coding
or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive …
or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive …
Integrating machine learning and genome editing for crop improvement
Genome editing is a promising technique that has been broadly utilized for basic gene
function studies and trait improvements. Simultaneously, the exponential growth of …
function studies and trait improvements. Simultaneously, the exponential growth of …