Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

The development of artificial metalloenzymes (ArMs) aims to expand the capabilities of
enzymatic catalysis, most notably towards new reaction mechanisms. Frequently, ArMs …

Transition-metal-catalyzed carbene insertion reactions of a nitrogen–hydrogen bond have
emerged as robust and versatile methods for the construction of C–N bonds. While …

Artificial photoenzymes with novel catalytic modes not found in nature are in high demand;
yet, they also present significant challenges in the field of biocatalysis. In this study, a …

Embedding a catalytically competent transition metal into a protein scaffold affords an
artificial metalloenzyme (ArM). Such hybrid catalysts display features that are reminiscent of …

Phosphine ligands are the most important class of ligands for cross-coupling reactions due
to their unique electronic and steric properties. However, metalloproteins generally rely on …

Therapeutic enzymes present excellent opportunities for the treatment of human disease,
modulation of metabolic pathways and system detoxification. However, current use of …

Squalene-hopene cyclases are a highly valuable and attractive class of membrane-bound
enzymes as sustainable biotechnological tools to produce aromas and bioactive …

The self-sufficient cytochrome P450 102A1 (P450BM3), known for its highly efficient
activation of molecular oxygen, was reconstituted with manganese protoporphyrin IX. This …

Alkylidene cyclopropanes (ACPs) are valuable synthetic intermediates because of their
constrained structure and opportunities for further diversification. Although routes to ACPs …