Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

Fast fit-free analysis of fluorescence lifetime imaging via deep learning

JT Smith, R Yao, N Sinsuebphon… - Proceedings of the …, 2019 - National Acad Sciences
Fluorescence lifetime imaging (FLI) provides unique quantitative information in biomedical
and molecular biology studies but relies on complex data-fitting techniques to derive the …

Deep learning in biomedical optics

L Tian, B Hunt, MAL Bell, J Yi, JT Smith… - Lasers in surgery …, 2021 - Wiley Online Library
This article reviews deep learning applications in biomedical optics with a particular
emphasis on image formation. The review is organized by imaging domains within …

Architecture and applications of a high resolution gated SPAD image sensor

S Burri, Y Maruyama, X Michalet, F Regazzoni… - Optics express, 2014 - opg.optica.org
We present the architecture and three applications of the largest resolution image sensor
based on single-photon avalanche diodes (SPADs) published to date. The sensor …

0.5 billion events per second time correlated single photon counting using CMOS SPAD arrays

N Krstajić, S Poland, J Levitt, R Walker, A Erdogan… - Optics letters, 2015 - opg.optica.org
We present a digital architecture for fast acquisition of time correlated single photon counting
(TCSPC) events from a 32× 32 complementary metal oxide semiconductor (CMOS) single …

Imaging proteins in vivo using fluorescence lifetime microscopy

F Festy, SM Ameer-Beg, T Ng, K Suhling - Molecular Biosystems, 2007 - pubs.rsc.org
Imaging proteins in vivo using fluorescence lifetime microscopy - Molecular BioSystems (RSC
Publishing) DOI:10.1039/B617204K Royal Society of Chemistry View PDF VersionPrevious …

Effect of refractive index on the fluorescence lifetime of green fluorescent protein

C Tregidgo, JA Levitt, K Suhling - Journal of biomedical optics, 2008 - spiedigitallibrary.org
The average fluorescence lifetime of the green fluorescent protein (GFP) in solution is a
function of the refractive index of its environment. We report that this is also the case for GFP …

High-frequency MHz-order vibration enables cell membrane remodeling and lipid microdomain manipulation

LA Ambattu, B Del Rosal, CE Conn, LY Yeo - Biophysical Journal, 2025 - cell.com
We elucidate the mechanism underpinning a recently discovered phenomenon in which
cells respond to MHz-order mechanostimuli. Deformations induced along the plasma …

Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis

L Liu, Q Yang, M Zhang, Z Wu, P Xue - Journal of Innovative Optical …, 2019 - World Scientific
Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding
microenvironment, and hence it can quantitatively reveal the physiological characterization …