Ultra-sensitive detection of ecologically rare fish from eDNA samples based on the RPA-CRISPR/Cas12a technology

XY Wei, L Liu, H Hu, HJ Jia, LK Bu, DS Pei - Iscience, 2023 - cell.com
Environmental DNA (eDNA) research holds great promise for improving biodiversity science
and conservation efforts by enabling worldwide species censuses in near real-time. Current …

Rapid detection of subterranean fauna from passive sampling of groundwater eDNA

M van der Heyde, J Alexander, P Nevill… - Environmental …, 2023 - Wiley Online Library
Groundwater is an essential source of freshwater that supports surface ecosystems as well
as organisms adapted to living underground. The impacts of anthropogenic climate change …

Large‐scale validation of 46 invasive species assays using an enhanced in silico framework

JA Kronenberger, TM Wilcox, MK Young… - Environmental …, 2024 - Wiley Online Library
The need for widespread occurrence data to inform species conservation has prompted
interest in large, national‐scale environmental DNA (eDNA) monitoring strategies. However …

Prey ration, temperature, and predator species influence digestion rates of prey DNA inferred from qPCR and metabarcoding

C Dick, WA Larson, K Karpan… - Molecular Ecology …, 2023 - Wiley Online Library
Diet analysis is a vital tool for understanding trophic interactions and is frequently used to
inform conservation and management. Molecular approaches can identify diet items that are …

Metabarcoding is (usually) more cost effective than seining or qPCR for detecting tidewater gobies and other estuarine fishes

K Lafferty - PeerJ, 2024 - peerj.com
Many studies have shown that environmental DNA (eDNA) sampling can be more sensitive
than traditional sampling. For instance, past studies found a specific qPCR probe of a water …

Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China

S Peng, L Wang, Y Ma, L Ye, C Hou, Y Liu… - Ecology and …, 2023 - Wiley Online Library
Frequently occurring jellyfish blooms have severe impacts on the socioeconomics of coastal
areas, which stress the importance of early detection and assessments of blooming jellyfish …

[HTML][HTML] Evaluating metabarcoding markers for identifying zooplankton and ichthyoplankton communities to species in the Salish Sea: Morphological comparisons and …

CA Stepien, HK Schultz, SM McAllister, EL Norton… - DNA, 2023 - mdpi.com
Zooplankton and ichthyoplankton community assessments depend on species diagnostics,
yet morphological identifications are time-consuming, require taxonomic expertise, and are …

Potential of a mammalian and avian environmental DNA metabarcoding assay as a tool for fecal pollution source tracking in river waters

A Hata, Y Meuchi, K Kuroda, K Sankoda… - … : Water Research & …, 2025 - pubs.rsc.org
Currently, common fecal source identification methods in water environments are based on
the detection of host-specific gene markers, such as 16S rRNA gene fractions of …

From jaguars to pathogens: simultaneous genetic detection of diverse taxa across biodiversity hotspots in the US arid southwest

MF Sleeting, TM Wilcox, DH Mason… - Conservation Genetics …, 2024 - Springer
The management of biodiversity on arid landscapes can be challenging: Arid landscapes
are often highly vulnerable to environmental and land-use changes. Moreover, these …

Assessment of Fish Species in Wanlv Lake, the Largest Drinking Water Source in South China, by Environmental DNA Metabarcoding Technology

J Wu, J Fu, D Zhou, J Huang, M Xu - Fishes, 2024 - mdpi.com
As the largest lake in South China, Wanlv Lake is also an important drinking water source for
Guangdong and Hong Kong and is responsible for water supplying of more than 40 million …