Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
[HTML][HTML] Identification of required host factors for SARS-CoV-2 infection in human cells
To better understand host-virus genetic dependencies and find potential therapeutic targets
for COVID-19, we performed a genome-scale CRISPR loss-of-function screen to identify …
for COVID-19, we performed a genome-scale CRISPR loss-of-function screen to identify …
A benchmark of algorithms for the analysis of pooled CRISPR screens
Genome-wide pooled CRISPR-Cas-mediated knockout, activation, and repression screens
are powerful tools for functional genomic investigations. Despite their increasing importance …
are powerful tools for functional genomic investigations. Despite their increasing importance …
Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute
Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR–Cas9) is a
powerful technology for the systematic evaluation of gene function. Statistically principled …
powerful technology for the systematic evaluation of gene function. Statistically principled …
In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target
Despite remarkable clinical efficacy of immune checkpoint blockade (ICB) in cancer
treatment, ICB benefits for triple-negative breast cancer (TNBC) remain limited. Through …
treatment, ICB benefits for triple-negative breast cancer (TNBC) remain limited. Through …
Unbiased transcription factor CRISPR screen identifies ZNF800 as master repressor of enteroendocrine differentiation
Enteroendocrine cells (EECs) are hormone-producing cells residing in the epithelium of
stomach, small intestine (SI), and colon. EECs regulate aspects of metabolic activity …
stomach, small intestine (SI), and colon. EECs regulate aspects of metabolic activity …
Selective TnsC recruitment enhances the fidelity of RNA-guided transposition
Bacterial transposons are pervasive mobile genetic elements that use distinct DNA-binding
proteins for horizontal transmission. For example, Escherichia coli Tn 7 homes to a specific …
proteins for horizontal transmission. For example, Escherichia coli Tn 7 homes to a specific …
QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis
INTRODUCTION Tissue homeostasis requires the coordinated activity of multiple cell types
to initiate and then resolve inflammation. Endoplasmic reticulum (ER) stress is a hallmark of …
to initiate and then resolve inflammation. Endoplasmic reticulum (ER) stress is a hallmark of …
Synthetic lethal and resistance interactions with BET bromodomain inhibitors in triple-negative breast cancer
BET bromodomain inhibitors (BBDIs) are candidate therapeutic agents for triple-negative
breast cancer (TNBC) and other cancer types, but inherent and acquired resistance to BBDIs …
breast cancer (TNBC) and other cancer types, but inherent and acquired resistance to BBDIs …
High-throughput PRIME-editing screens identify functional DNA variants in the human genome
Despite tremendous progress in detecting DNA variants associated with human disease,
interpreting their functional impact in a high-throughput and single-base resolution manner …
interpreting their functional impact in a high-throughput and single-base resolution manner …