3D electron diffraction: the nanocrystallography revolution
Crystallography of nanocrystalline materials has witnessed a true revolution in the past 10
years, thanks to the introduction of protocols for 3D acquisition and analysis of electron …
years, thanks to the introduction of protocols for 3D acquisition and analysis of electron …
The cryo-EM method microcrystal electron diffraction (MicroED)
In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …
The CryoEM method MicroED as a powerful tool for small molecule structure determination
In the many scientific endeavors that are driven by organic chemistry, unambiguous
identification of small molecules is of paramount importance. Over the past 50 years, NMR …
identification of small molecules is of paramount importance. Over the past 50 years, NMR …
Cryo-EM structure of an amyloid fibril formed by full-length human prion protein
LQ Wang, K Zhao, HY Yuan, Q Wang, Z Guan… - Nature Structural & …, 2020 - nature.com
Prion diseases are caused by the misfolding of prion protein (PrP). Misfolded PrP forms
protease-resistant aggregates in vivo (PrPSc) that are able to template the conversion of the …
protease-resistant aggregates in vivo (PrPSc) that are able to template the conversion of the …
Secondary forces in protein folding
A complete inventory of the forces governing protein folding is critical for productive protein
modeling, including structure prediction and de novo design, as well as understanding …
modeling, including structure prediction and de novo design, as well as understanding …
MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography
MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory
cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms …
cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms …
A close look at molecular self-assembly with the transmission electron microscope
Molecular self-assembly is pervasive in the formation of living and synthetic materials.
Knowledge gained from research into the principles of molecular self-assembly drives …
Knowledge gained from research into the principles of molecular self-assembly drives …
General principles underpinning amyloid structure
AIP Taylor, RA Staniforth - Frontiers in Neuroscience, 2022 - frontiersin.org
Amyloid fibrils are a pathologically and functionally relevant state of protein folding, which is
generally accessible to polypeptide chains and differs fundamentally from the globular state …
generally accessible to polypeptide chains and differs fundamentally from the globular state …
Cryo-EM structure of a human prion fibril with a hydrophobic, protease-resistant core
Self-templating assemblies of the human prion protein are clinically associated with
transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a …
transmissible spongiform encephalopathies. Here we present the cryo-EM structure of a …
Pathologic polyglutamine aggregation begins with a self-poisoning polymer crystal
A long-standing goal of amyloid research has been to characterize the structural basis of the
rate-determining nucleating event. However, the ephemeral nature of nucleation has made …
rate-determining nucleating event. However, the ephemeral nature of nucleation has made …