Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
A guide to fluorescent protein FRET pairs
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …
A highly photostable and bright green fluorescent protein
M Hirano, R Ando, S Shimozono, M Sugiyama… - Nature …, 2022 - nature.com
The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …
Improving FRET dynamic range with bright green and red fluorescent proteins
A variety of genetically encoded reporters use changes in fluorescence (or Förster)
resonance energy transfer (FRET) to report on biochemical processes in living cells. The …
resonance energy transfer (FRET) to report on biochemical processes in living cells. The …
Intravital imaging reveals how BRAF inhibition generates drug-tolerant microenvironments with high integrin β1/FAK signaling
E Hirata, MR Girotti, A Viros, S Hooper… - Cancer cell, 2015 - cell.com
Intravital imaging of BRAF-mutant melanoma cells containing an ERK/MAPK biosensor
reveals how the tumor microenvironment affects response to BRAF inhibition by PLX4720 …
reveals how the tumor microenvironment affects response to BRAF inhibition by PLX4720 …
High-sensitivity measurements of multiple kinase activities in live single cells
Increasing evidence has shown that population dynamics are qualitatively different from
single-cell behaviors. Reporters to probe dynamic, single-cell behaviors are desirable yet …
single-cell behaviors. Reporters to probe dynamic, single-cell behaviors are desirable yet …
ERK-mediated mechanochemical waves direct collective cell polarization
During collective migration of epithelial cells, the migration direction is aligned over a tissue-
scale expanse. Although the collective cell migration is known to be directed by mechanical …
scale expanse. Although the collective cell migration is known to be directed by mechanical …
Frequency-modulated pulses of ERK activity transmit quantitative proliferation signals
Summary The EGF-stimulated ERK/MAPK pathway is a key conduit for cellular proliferation
signals and a therapeutic target in many cancers. Here, we characterize two central …
signals and a therapeutic target in many cancers. Here, we characterize two central …
Accurate information transmission through dynamic biochemical signaling networks
Stochasticity inherent to biochemical reactions (intrinsic noise) and variability in cellular
states (extrinsic noise) degrade information transmitted through signaling networks. We …
states (extrinsic noise) degrade information transmitted through signaling networks. We …
Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling
B Ponsioen, JB Post, JR Buissant des Amorie… - Nature cell …, 2021 - nature.com
Direct targeting of the downstream mitogen-activated protein kinase (MAPK) pathway to
suppress extracellular-regulated kinase (ERK) activation in KRAS and BRAF mutant …
suppress extracellular-regulated kinase (ERK) activation in KRAS and BRAF mutant …