Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
Fluorescence resonance energy transfer at the single-molecule level
Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Deep-LASI: deep-learning assisted, single-molecule imaging analysis of multi-color DNA origami structures
Single-molecule experiments have changed the way we explore the physical world, yet data
analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI …
analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI …
The fork protection complex recruits FACT to reorganize nucleosomes during replication
Chromosome replication depends on efficient removal of nucleosomes by accessory factors
to ensure rapid access to genomic information. Here, we show this process requires …
to ensure rapid access to genomic information. Here, we show this process requires …
Small RNAs and Hfq capture unfolded RNA target sites during transcription
Small ribonucleoproteins (sRNPs) target nascent precursor RNAs to guide folding,
modification, and splicing during transcription. Yet, rapid co-transcriptional folding of the …
modification, and splicing during transcription. Yet, rapid co-transcriptional folding of the …
Unifying mechanism for thiol-induced photoswitching and photostability of cyanine dyes
Cyanines (Cy3, Cy5, Cy3B) are the most utilized dyes for single-molecule fluorescence and
localization-based super-resolution imaging. These modalities exploit cyanines' versatile …
localization-based super-resolution imaging. These modalities exploit cyanines' versatile …
Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels
Multiplexed, real-time fluorescence detection at the single-molecule level can reveal the
stoichiometry, dynamics and interactions of multiple molecular species in mixtures and other …
stoichiometry, dynamics and interactions of multiple molecular species in mixtures and other …
[HTML][HTML] An integrated transport mechanism of the maltose ABC importer
R Mächtel, A Narducci, DA Griffith, T Cordes… - Research in …, 2019 - Elsevier
ATP-binding cassette (ABC) transporters use the energy of ATP hydrolysis to transport a
large diversity of molecules actively across biological membranes. A combination of …
large diversity of molecules actively across biological membranes. A combination of …
Sequence-specific dynamic DNA bending explains mitochondrial TFAM's dual role in DNA packaging and transcription initiation
Mitochondrial transcription factor A (TFAM) employs DNA bending to package mitochondrial
DNA (mtDNA) into nucleoids and recruit mitochondrial RNA polymerase (POLRMT) at …
DNA (mtDNA) into nucleoids and recruit mitochondrial RNA polymerase (POLRMT) at …