Expanding the catalytic landscape of metalloenzymes with lytic polysaccharide monooxygenases

A Munzone, VGH Eijsink, JG Berrin… - Nature Reviews …, 2024 - nature.com
Lytic polysaccharide monooxygenases (LPMOs) have an essential role in global carbon
cycle, industrial biomass processing and microbial pathogenicity by catalysing the oxidative …

A conserved second sphere residue tunes copper site reactivity in lytic polysaccharide monooxygenases

KR Hall, C Joseph, I Ayuso-Fernández… - Journal of the …, 2023 - ACS Publications
Lytic polysaccharide monooxygenases (LPMOs) are powerful monocopper enzymes that
can activate strong C–H bonds through a mechanism that remains largely unknown. Herein …

Reductants fuel lytic polysaccharide monooxygenase activity in a pH‐dependent manner

O Golten, I Ayuso‐Fernández, KR Hall… - FEBS …, 2023 - Wiley Online Library
Polysaccharide‐degrading mono‐copper lytic polysaccharide monooxygenases (LPMOs)
are efficient peroxygenases that require electron donors (reductants) to remain in the active …

Insight into the peroxygenase activity of lytic polysaccharide monooxygenases (LPMO): Recent progress and mechanistic understanding

W Gao, H Yin - Chemical Physics Reviews, 2023 - pubs.aip.org
The discovery of lytic polysaccharide monooxygenases (LPMOs) as monocopper enzymes
for the oxidative cleavage of glycosidic bonds in recalcitrant polysaccharides has …

On the pH Dependency of the Catalysis by a Lytic Polysaccharide Monooxygenase from the Fungus Trichoderma reesei

S Kuusk, M Lipp, S Mahajan, P Väljamäe - ACS Catalysis, 2024 - ACS Publications
Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of
glycosidic bonds in industrially important polysaccharides like cellulose. The activity of these …

LPMO‐like activity of bioinspired copper complexes: from model substrate to extended polysaccharides

R Leblay, R Delgadillo‐Ruíz, C Decroos… - …, 2023 - Wiley Online Library
Polysaccharide oxidative depolymerization is highly desirable to achieve recalcitrant
biomass valorization. Inspired by recently discovered Lytic Polysaccharide …

Integrated Experimental and Theoretical Investigation of Copper Active Site Properties of a Lytic Polysaccharide Monooxygenase from Serratia marcescens

A Munzone, M Pujol, A Tamhankar, C Joseph… - Inorganic …, 2024 - ACS Publications
In this paper, we employed a multidisciplinary approach, combining experimental
techniques and density functional theory (DFT) calculations to elucidate key features of the …

Selective oxidation of active site aromatic residues in engineered Cu proteins

KS Uyeda, AH Follmer, AS Borovik - Chemical Science, 2025 - pubs.rsc.org
Recent studies have revealed critical roles for the local environments surrounding
metallocofactors, such as the newly identified CuD site in particulate methane …