The growing population increases the need to develop advanced biological methods for
utilizing renewable and sustainable resources to produce environmentally friendly biofuels …

Highlights•Directed evolution allows complex trait engineering at a systems-scale.•Synthetic
biology accelerates directed evolution with diversity generation tools.•Synthetic biology can …

Microbes are capable of producing alcohols, making them an important source of alternative
energy that can replace fossil fuels. However, these alcohols can be toxic to the microbes …

Background Clostridium acetobutylicum is a model organism for both clostridial biology and
solvent production. The organism is exposed to its own toxic metabolites butyrate and …

Fuels and polymer precursors are widely used in daily life and in many industrial processes.
Although these compounds are mainly derived from petrol, bacteria and yeast can produce …

The concept, science, and technology of biofuels has captured the public's imagination as
well as the attention of the academic and industrial R&D communities over the last 10 years …

A major challenge in producing chemicals and biofuels is to increase the tolerance of the
host organism to toxic products or byproducts. An Escherichia coli strain with superior …

Background N-Butanol has favorable characteristics for use as either an alternative fuel or
platform chemical. Bio-based n-butanol production using microbes is an emerging …

Highlights•This review covers recent methods for strain development.•New techniques of
discovery enabled by the advent of high throughput technologies.•Rational design builds on …

The growing need to address current energy and environmental problems has sparked an
interest in develo** improved biological methods to produce liquid fuels from renewable …