Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …

Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and
microscope that uses a plane of light to optically section and view tissues with subcellular …

The visualization of whole organs and organisms through tissue clearing and fluorescence
volumetric imaging has revolutionized the way we look at biological samples. Its application …

Laser beams that can self-reconstruct their initial beam profile even in the presence of
massive phase perturbations are able to propagate deeper into inhomogeneous media. This …

A key requirement for performing three-dimensional (3D) imaging using optical microscopes
is that they be capable of optical sectioning by distinguishing in-focus signal from out-of …