Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation

CK Park, NC Horton - Biophysical reviews, 2019 - Springer
Filament formation by non-cytoskeletal enzymes has been known for decades, yet only
relatively recently has its wide-spread role in enzyme regulation and biology come to be …

Structures, mechanisms, and kinetic advantages of the SgrAI filament forming mechanism

NC Horton, D Lyumkis - Critical Reviews in Biochemistry and …, 2024 - Taylor & Francis
This review documents investigations leading to the unprecedented discovery of
filamentation as a mode of enzyme regulation in the type II restriction endonuclease SgrAI …

DNA binding and cleavage by the human parvovirus B19 NS1 nuclease domain

JL Sanchez, Z Romero, A Quinones, KR Torgeson… - Biochemistry, 2016 - ACS Publications
Infection with human parvovirus B19 (B19V) has been associated with a myriad of illnesses,
including erythema infectiosum (Fifth disease), hydrops fetalis, arthropathy, hepatitis, and …

Mechanism of filamentation-induced allosteric activation of the SgrAI endonuclease

S Polley, D Lyumkis, NC Horton - Structure, 2019 - cell.com
Filament formation by enzymes is increasingly recognized as an important phenomenon
with potentially unique regulatory properties and biological roles. SgrAI is an allosterically …

DNA Sequence Control of Enzyme Filamentation and Activation of the SgrAI Endonuclease

N Ghadirian, RD Morgan, NC Horton - Biochemistry, 2024 - ACS Publications
Enzyme polymerization (also known as filamentation) has emerged as a new layer of
enzyme regulation. SgrAI is a sequence-dependent DNA endonuclease that forms …

Structural analysis of activated SgrAI–DNA oligomers using ion mobility mass spectrometry

X Ma, S Shah, M Zhou, CK Park, VH Wysocki… - Biochemistry, 2013 - ACS Publications
SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence
and exhibits self-modulation of DNA cleavage activity and sequence specificity. Previous …

The role of filamentation in activation and DNA sequence specificity of the sequence-specific endonuclease SgrAI

D Lyumkis, NC Horton - Biochemical Society Transactions, 2022 - portlandpress.com
Filament formation by metabolic, biosynthetic, and other enzymes has recently come into
focus as a mechanism to fine-tune enzyme activity in the cell. Filamentation is key to the …

Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization

D Lyumkis, H Talley, A Stewart, S Shah, CK Park… - Structure, 2013 - cell.com
SgrAI is a sequence specific DNA endonuclease that functions through an unusual
enzymatic mechanism that is allosterically activated 200-to 500-fold by effector DNA, with a …

Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity

Z Shan, N Ghadirian, D Lyumkis, NC Horton - Journal of Biological …, 2022 - ASBMB
Enzyme filamentation is a widespread phenomenon that mediates enzyme regulation and
function. For the filament-forming sequence-specific DNA endonuclease SgrAI, the process …

Zinc enhances adiponectin oligomerization to octadecamers but decreases the rate of disulfide bond formation

DB Briggs, RM Giron, K Schnittker, MV Hart, CK Park… - Biometals, 2012 - Springer
Adiponectin, a hormone secreted from adipocytes, has been shown to protect against
development of insulin resistance, ischemia–reperfusion injury, and inflammation …