Origins and evolution of CRISPR-Cas systems
EV Koonin, KS Makarova - Philosophical Transactions of …, 2019 - royalsocietypublishing.org
CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex
machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic …
machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic …
Delivering CRISPR: a review of the challenges and approaches
CA Lino, JC Harper, JP Carney, JA Timlin - Drug delivery, 2018 - Taylor & Francis
Gene therapy has long held promise to correct a variety of human diseases and defects.
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
Applications of CRISPR technologies in research and beyond
R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …
Methodologies for improving HDR efficiency
M Liu, S Rehman, X Tang, K Gu, Q Fan, D Chen… - Frontiers in …, 2019 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …
(Cas9) is a precise genome manipulating technology that can be programmed to induce …
An updated evolutionary classification of CRISPR–Cas systems
KS Makarova, YI Wolf, OS Alkhnbashi… - Nature reviews …, 2015 - nature.com
The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
[HTML][HTML] Biology and applications of CRISPR systems: harnessing nature's toolbox for genome engineering
Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
A genomic catalogue of soil microbiomes boosts mining of biodiversity and genetic resources
Soil harbors a vast expanse of unidentified microbes, termed as microbial dark matter,
presenting an untapped reservoir of microbial biodiversity and genetic resources, but has …
presenting an untapped reservoir of microbial biodiversity and genetic resources, but has …
Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems
BACKGROUND Prokaryotes have evolved multiple systems to combat invaders such as
viruses and plasmids. Examples of such defense systems include receptor masking …
viruses and plasmids. Examples of such defense systems include receptor masking …
CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria
DNA is an excellent medium for archiving data. Recent efforts have illustrated the potential
for information storage in DNA using synthesized oligonucleotides assembled in vitro,,,,,. A …
for information storage in DNA using synthesized oligonucleotides assembled in vitro,,,,,. A …