Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

C Jo, J Zhang, JM Tam, GM Church, AS Khalil… - Materials Today Bio, 2023 - Elsevier
Filamentous fungi drive carbon and nutrient cycling across our global ecosystems, through
its interactions with growing and decaying flora and their constituent microbiomes. The …

Filamentous fungi for the production of enzymes, chemicals and materials

HAB Wösten - Current opinion in biotechnology, 2019 - Elsevier
Highlights•Fungi can be used as a cell factory and for production of mycelium
materials.•Monofungal and mixed fungal cultures produce different profiles of secreted …

A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger

N Paege, S Jung, P Schäpe, D Müller-Hagen… - PLoS …, 2016 - journals.plos.org
Understanding the genetic, molecular and evolutionary basis of cysteine-stabilized
antifungal proteins (AFPs) from fungi is important for understanding whether their function is …

The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production

D Aerts, EE Hauer, RA Ohm, M Arentshorst… - Antonie Van …, 2018 - Springer
Aspergillus niger secretes proteins throughout the colony except for the zone that forms
asexual spores called conidia. Inactivation of flbA that encodes a regulator of G-protein …

[HTML][HTML] Functional analysis of FlbA-regulated transcription factor genes in Aspergillus niger using a multiplexed CRISPRoff system

X Chen, JPM Torres, SVK Tedjai, LG Lugones… - International Journal of …, 2024 - Elsevier
FlbA of Aspergillus niger (indirectly) regulates 36 transcription factor (TF) genes. As a result,
it promotes sporulation and represses vegetative growth, protein secretion and lysis. In this …

[HTML][HTML] The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

X Chen, JPM Torres, PJ Vonk, JMA Damen… - Fungal Genetics and …, 2024 - Elsevier
Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis,
abolished sporulation, and an increased secretome complexity. A total of 36 transcription …

FluG affects secretion in colonies of Aspergillus niger

F Wang, P Krijgsheld, M Hulsman, C de Bekker… - Antonie Van …, 2015 - Springer
Colonies of Aspergillus niger are characterized by zonal heterogeneity in growth,
sporulation, gene expression and secretion. For instance, the glucoamylase gene glaA is …

FlbA-Regulated Gene rpnR Is Involved in Stress Resistance and Impacts Protein Secretion when Aspergillus niger Is Grown on Xylose

D Aerts, SG van den Bergh, H Post… - Applied and …, 2019 - Am Soc Microbiol
Proteins are secreted throughout the mycelium of Aspergillus niger except for the
sporulating zone. A link between sporulation and repression of protein secretion was …

Involvement of a G Protein Regulatory Circuit in Alternative Oxidase Production in Neurospora crassa

N Bosnjak, KM Smith, I Asaria… - G3: Genes …, 2019 - academic.oup.com
The Neurospora crassa nuclear aod-1 gene encodes an alternative oxidase that functions in
mitochondria. The enzyme provides a branch from the standard electron transport chain by …

Effects of different carbon sources on the oxidative stress tolerance of Aspergillus niger HY2 isolated from spoiled paddies

S Tan, Y Xu, H Wu, A Niu, X Yin… - FEMS Microbiology …, 2022 - academic.oup.com
Fungi often experience oxidative stress in response to the environment during growth. In this
study, Aspergillus niger HY2, whose presence easily results in paddy mildew, was used to …