An overview of biological macromolecule crystallization

IR Krauss, A Merlino, A Vergara, F Sica - International journal of …, 2013 - mdpi.com
The elucidation of the three dimensional structure of biological macromolecules has
provided an important contribution to our current understanding of many basic mechanisms …

The non-classical nucleation of crystals: microscopic mechanisms and applications to molecular crystals, ice and calcium carbonate

RP Sear - International Materials Reviews, 2012 - Taylor & Francis
Crystals form via nucleation followed by growth. Often nucleation data are interpreted using
the classical theory of nucleation, which is essentially a simple theory for the nucleation of a …

Molecular basis for the recognition of methylated adenines in RNA by the eukaryotic YTH domain

S Luo, L Tong - Proceedings of the National Academy of …, 2014 - National Acad Sciences
Methylation of the N6 position of selected internal adenines (m6A) in mRNAs and noncoding
RNAs is widespread in eukaryotes, and the YTH domain in a collection of proteins …

Surface-induced crystallization of pharmaceuticals and biopharmaceuticals: A review

F Artusio, R Pisano - International journal of pharmaceutics, 2018 - Elsevier
Despite the wide occurrence of crystallization in the pharmaceutical industry, deep
understanding and fine control of the process remain a tricky issue. Nevertheless, the …

Porous nucleating agents for protein crystallization

S Khurshid, E Saridakis, L Govada, NE Chayen - Nature protocols, 2014 - nature.com
Solving the structure of proteins is pivotal to achieving success in rational drug design and in
other biotechnological endeavors. The most powerful method for determining the structure of …

Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence

S Engilberge, F Riobé, S Di Pietro, L Lassalle… - Chemical …, 2017 - pubs.rsc.org
Macromolecular crystallography suffers from two major issues: getting well-diffracting
crystals and solving the phase problem inherent to large macromolecules. Here, we …

Towards a 'universal'nucleant for protein crystallization

E Saridakis, NE Chayen - Trends in biotechnology, 2009 - cell.com
The expression 'crystal clear'stems from the science of crystallography, which determines
the detailed atomic structures of materials by exposing crystals to X-rays. Protein structures …

Random microseeding: a theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization

PD Shaw Stewart, SA Kolek, RA Briggs… - Crystal Growth & …, 2011 - ACS Publications
Microseed matrix-screening combined with random screens (rMMS) is a significant recent
breakthrough in protein crystallization. In this study, a very reproducible assay for crystal …

Advancements (and challenges) in the study of protein crystal nucleation and growth; thermodynamic and kinetic explanations and comparison with small-molecule …

CN Nanev - Progress in Crystal Growth and Characterization of …, 2020 - Elsevier
This paper reviews advancements and some novel ideas (not yet covered by reviews and
monographs) concerning thermodynamics and kinetics of protein crystal nucleation and …

Protein crystal nucleation in pores

CN Nanev, E Saridakis, NE Chayen - Scientific reports, 2017 - nature.com
The most powerful method for protein structure determination is X-ray crystallography which
relies on the availability of high quality crystals. Obtaining protein crystals is a major …